Table 1 Enzyme metabolic rates, depending on the concentration of product produced, comparing immortalised HepaRG cells against iPSC-Hep cell lines [14], showing the different systems responding to a known compound over 8 and 29 days. IPSCs responded in a far more stable manner over 29 days, as HepaRG cells metabolic rates were too low to measure
Cell modelDayCell per wellDrug oxidation activity
pmol products/hour/1 × 105 cells
CYP1A2CYP2C9CYP2C19CYP2D6CYP3A4
HepaRG81.80.93 ± 0.030.040 ± 0.0044.1 ± 1.011 ± 2.020 ± 2.0
iPSC #181.50.93 ± 0.240.004 ± 0.0011.2 ± 0.207.0 ± 2.094 ± 20.0
291.11.2 ± 0.100.053 ± 0.0067.9 ± 0.8024 ± 4.026 ± 2.0
iPSC #281.50.78 ± 0.190.002 ± 0.00041.0 ± 0.104.4 ± 1.177 ± 6.0
291.31.2 ± 0.100.027 ± 0.0056.5 ± 0.7016 ± 1.024 ± 3.0