Table 1 Kinetics parameters for conversion of (2S)-Nε-trimethyllysine to (2S,3S)-3-hydroxy-Nε-trimethyllysine by TMLH and its variants
(2S)-Nε-trimethyllysineVmax (µM min−1)KM (mM)
WT TMLH147 ± 131.18 ± 0.13
W221F77 ± 9.01.47 ± 0.31
T269A72 ± 4.00.70 ± 0.09
D244E12 ± 0.750.32 ± 0.07
2OG  
 WT TMLH114 ± 90.42 ± 0.12
 W221F35 ± 30.35 ± 0.11
 T269A122 ± 201.97 ± 0.70
 D244E11 ± 0.61.12 ± 0.16