Table 2 MRM transitions used for detecting the prostaglandin species and their retention times

Chromatograms of the 10 min chromatographic separations are provided in Supplementary Figure S1.

ProstaglandinMRM transitionRetention time (min)
6-keto PGF369.2 > 163.13.09
Iso-PGF353.0 > 192.94.25
PGF353.0 > 192.94.88
PGF355.2 > 311.24.96
PGE2351.1 > 271.15.06
PGE1353.2 > 317.25.31