Table 5 Kinetic characterisation of trypanosomatid recombinant aldo-keto reductases with various substrates
SubstrateUnitsTcAKRLiPGFS1LiPGFS2TbPGFS
Glyoxal
 kcats14.56 ± 0.057.31 ± 0.191.05 ± 0.02ND
Embedded Image1mM8.30 ± 0.3136.1 ± 2.390.56 ± 0.05ND
Embedded ImageM−1 s−15492021880ND
Methylglyoxal
 kcats−14.69 ± 0.038.72 ± 0.171.13 ± <0.010.38 ± 0.007
Embedded ImagemM0.24 ± <0.0010.80 ± 0.070.02 ± <0.0010.01 ± <0.001
Embedded ImageM−1 s−119 50010 90056 50038 000
Phenylglyoxal
 kcats−14.46 ± 0.0810.4 ± 0.221.17 ± 0.020.34 ± 0.014
Embedded ImagemM0.05 ± 0.0030.14 ± 0.0080.01 ± 0.00090.005 ± 0.0008
Embedded ImageM−1 s−189 20074 30058 50068 000
2-Nitrobenzaldehyde
 kcats−12.25 ± 0.1128.50 ± 0.211.04 ± 0.020.41 ± 0.002
Embedded ImagemM0.71 ± 0.0820.32 ± 0.020.0024 ± 0.00030.003 ± 0.0003
Embedded ImageM−1 s−1317026 600433 000140 000
3-Nitrobenzaldehyde
 kcats−14.07 ± 0.171.28 ± 0.031.04 ± 0.020.34 ± 0.005
Embedded ImagemM2.20 ± 0.161.55 ± 0.060.002 ± 0.00030.002 ± 0.0001
Embedded ImageM−1 s−11850830520 000170 000
4-Nitrobenzaldehyde
 kcats−13.7 ± 0.150.48 ± 0.011.13 ± 0.0520.43 ± 0.008
Embedded ImagemM68 ± 0.070.43 ± 0.0260.004 ± 0.000620.0043 ± 0.0002
Embedded ImageM−1 s−154401 110283 000100 000
d,l-glyceraldehyde
 kcats−14.94 ± 0.118.72 ± 0.1122.46 ± 0.1320.37 ± 0.009
Embedded ImagemM1.50 ± 0.172.87 ± 0.0821.07 ± 0.1520.07 ± 0.005
Embedded ImageM−1 s−13290304023005290

Abbreviations: ND, not determined.

  • 1 Embedded Image values determined with 100 µM NADPH.

  • 2 Weighted mean of two individual replicates, all others are n ≥ 3.