Table 3 A survey of purified recombinant trypanosomatid aldo-keto reductases with various substrates

Compounds (60 µM) were assayed spectrophotometrically in the presence of 100 µM NADPH. Specific activity is given in µmol min−1 mg−1. Values are the means and standard deviations (n = 3).

SubstrateTcAKRLiPGFS1LiPGFS2TbPGFS
2-Nitrobenzaldehyde0.20 ± 0.0041.40 ± 0.042.51 ± 0.0290.73 ± 0.017
3-Nitrobenzaldehyde0.16 ± 0.0030.06 ± 0.0022.40 ± 0.0371.86 ± 0.009
4-Nitrobenzaldehyde0.39 ± 0.0090.06 ± 0.0022.49 ± 0.1420.71 ± 0.013
Glyoxal0.001 ± 0.0010.02 ± 0.0010.09 ± 0.0010.15 ± 0.005
Methylglyoxal1.20 ± 0.0220.61 ± 0.021.79 ± 0.0090.67 ± 0.012
Phenylglyoxal3.66 ± 0.0923.11 ± 0.0182.46 ± 0.0840.78 ± 0.037
Vitamin K3 epoxideN.A.N.A.N.D.N.A.
Glyceraldehyde0.05 ± 0.005N.D.0.16 ± 0.0020.13 ± 0.004
BenznidazoleN.A.N.A.N.A.N.A.

Abbreviations: NA, not active; ND, not determined.