Retention time (min) | Structure | Metabolite number^{1} | [M+H]^{+} | Ionisation state | WT lysate | NTR^{OE} Lys B | Control | TcAKR |
---|---|---|---|---|---|---|---|---|

3.1 | Benznidazole | NA | 261.10 | 1 | NA | NA | NA | NA |

1.59 | Hydroxylamine or hydroxy derivative 1 or 2 | 11 or 19 | 247.11 | 1 | Y | Y | N | N |

1.8 | Amino derivative of benznidazole | 1 | 231.12 | 1 | Y | Y | N | N |

1.49/1.59 | Dihydroxy-dihydro derivative | 3 | 265.13 | 1 | Y | Y | N | N |

1.59 | N-benzyl-2-guanidinoacetamide | 5 | 207.13 | 1 | N | Y | N | N |

1.3 | 2-Amino-N-benzylacetamide | 22 | 165.10 | 1 | Y | Y | N | N |

1.74 | Amino derivative + glutathione (isomer 1) | 7 or 8 | 536.19 | 1 | Y | Y | N | N |

268.56 | 2 | Y | Y | N | N | |||

1.83 | Amino derivative + glutathione (isomer 2) | 7 or 8 | 536.19 | 1 | Y | Y | N | N |

268.56 | 2 | Y | Y | N | N | |||

1.55/1.64 | Amino derivative + glutathione + H_{2}O | 4 | 554.20 | 1 | Y | Y | N | N |

277.60 | 2 | Y | Y | N | N | |||

1.76/1.93/2.31 | Amino derivative + cysteine | 9 | 350.13 | 1 | Y | Y | NN | NN |

1.34 | Amino derivative + ovothiol A | 12 or 14 | 430.17 | 1 | Y | Y | N | N |

215.58 | 2 | Y | Y | N | N | |||

3.8 | Possible benznidazole adduct | 26 | 385.18 | 1 | Y | Y | N | N |

1.63 | Possible benznidazole adduct | 28 | 368.12 | 1 | N | Y | N | N |

3.64/3.8 | Possible benznidazole adduct | NA | 614.35 | 1 | Y | Y | N | N |

2.13 | Possible benznidazole metabolite | NA | 204.05 | 1 | Y | Y | N | N |

Abbreviations: NA, not applicable.

↵1 The metabolite numbers refer to the metabolite list in Trochine et al. [10].