Table 2 Equilibrium dissociation constants for 991 binding to AMPK complexes

AMPK complexes were expressed in E. coli and dissociation constants (Kd) for 991 were determined using circular dichroism (CD). The Kd values are reported as the mean (±SEM) determined from at least three independent experiments. The values for the phosphorylated Thr172 complexes (P) are taken from a previous study (Xiao et al. [9]).

AMPK complexCD (306 nm) Kd (µM)
α1β1γ10.13 ± 0.09
α1β1γ1(P)0.08 ± 0.02
α2β1γ10.17 ± 0.03
α2β1γ1(P)0.09 ± 0.02