Table 3 Analysis of monomer to dimer proportions for the unoccupied and EGF-stimulated EGFR estimated at different statistical stringency thresholds.
Cell lineTreatmentQuaternary structure1.5× standard deviations2× standard deviations
EGFR-mEGFPUTMonomers79.2% [<1.48 MEU]85% [<1.64 MEU]
Dimers20.8% [>1.48 MEU]15% [>1.64 MEU]
Oligomers0% [>3.00 MEU]0% [>3.33 MEU]
EGFR-mEGFP100 nM EGF 15 minMonomers10.8%20.8%
Dimers87.1%78.3%
Oligomers2.1%0.8%
EGFR Y251A,R285S-mEGFPUTMonomers94.2%96.7%
Dimers5.4%2.9%
Oligomers0.4%0.4%
EGFR Y251A,R285S-mEGFP100 nM EGF
15 min
Monomers91.7%96.7%
Dimers8.3%3.3%
Oligomers0%0%

Abbreviation: UT, untreated.