Table 2 kcat/Km values determined in the present study
GTPase/GEFLiposomeskcat/Km (×105 M−1 s−1)
myrArf1/ARNO+130.1
His-Arf1/ARNO+52.1
myrArf6/ARNO+65.6
His-Arf6/ARNO+13.5
RhoG-His/Trio1.9
RhoG-His/Trio+59.1
Rac1-His/Trio0.9
Rac1-His/Trio+8.2
Rab1-His/DrrA8.5
Rab1-His/DrrA+3.4