Table 1 Midpoint denaturation temperatures (Tm1 and Tm2) for the melting transitions of hArc, measured by thermal-dependent CD and derivatization of the transitions

hArc (4.4 μM) was prepared in 10 mM potassium phosphate at the indicated pH and conditions.

ConditionTm1 (°C)Tm2 (°C)
pH 6.343.2±0.975.0±0.6
pH 7.446.4±1.174.3±0.2
pH 8.248.5±0.173.8±1.0
pH 7.4, 150 mM KF43.0±0.275.8±1.5
pH 7.4, 300 mM KF42.7±0.477.6±0.1
pH 7.4, 5 μg/ml heparin53.0±0.873.9±0.5
pH 7.4, 20 μM calcium46.5±0.974.3±0.5