Table 1 In vitro reduction of benzamidoxime by recombinantly expressed hmARC isoforms

A complete incubation mixture consisted of 10 μg of hmARC, 200 pmol of cyt b5, 20 pmol of cyt b5 reductase, 1 mM NADH and 0.5 mM benzamidoxime in 150 μl of 20 mM Mes buffer, pH 6.0. Results are means±S.D. for three different incubations each analysed twice via HPLC.

mARC isoformComposition of systemSpecific activity (nmol·min−1·mg−1 of total protein)
Recombinant hmARC1Complete34.3±0.9
Without cyt b515.4±2.3
Without cyt b5 reductase1.6±0.2
Without hmARC12.2±0.4
Only hmARC12.2±0.4
Without NADH0.2±0.1
Recombinant hmARC2Complete90.7±5.6
Without cyt b50.9±0.1
Without cyt b5 reductase1.2±0.2
Without hmARC20.3±0.1
Only hmARC20.2±0.1
Without NADH0.1±0.1