Table 2 A list of the most prevalent Rho GTPase GEFs, GAPs and GDIs in human platelets with their known target GTPases

The list was based on Takai et al. [147] augmented with information from domain homology searches conducted using protein sequence (www.uniprot.org/) and protein interaction (string-db.org/) databases. Table entries were cross-referenced against two recent proteomic databases such that only proteins known to be expressed are listed [145,146], and then ranked using mRNA transcript levels from human platelets [35].

UniProt IDGene nameProtein nameGTPase target
Q9NZN5ARHGEF12Rho guanine nucleotide exchange factor 12RhoA, RhoB, Rac
Q5JSP0FGD3FYVE, RhoGEF and PH domain-containing protein 3Cdc42
Q92888ARHGEF1Rho guanine nucleotide exchange factor 1RhoA
Q9NR81ARHGEF3Rho guanine nucleotide exchange factor 3RhoA, RhoB, Cdc42
O60229KALRNKalirinRac
Q8TCU6PREX1P-Rex1 (PtdIns(3,4,5)-dependent Rac exchanger 1)Rac
Q9UKW4VAV3Guanine nucleotide exchange factor VAV3Rac, Cdc42
O75962TRIOTriple functional domain protein (TRIO)Rac, RhoA
Q15052ARHGEF6Rho guanine nucleotide exchange factor 6Rac, Cdc42
Q8NF50DOCK8Dedicator of cytokinesis protein 8Rac, Cdc42
Q6ZSZ5ARHGEF18Rho guanine nucleotide exchange factor 18RhoB, Rac
Q9H7D0DOCK5Dedicator of cytokinesis protein 5RhoA, Rac
Q92974ARHGEF2Rho guanine nucleotide exchange factor 2RhoA
Q07889SOS1Son of sevenless homologue 1 (SOS-1)Rac
Q92608DOCK2Dedicator of cytokinesis protein 2Rac
P15498VAV1Proto-oncogene VavRac, Cdc42
Q9NZM3ITSN2Intersectin-2Cdc42
Q5T5U3ARHGAP21Rho GTPase-activating protein 21RhoA, Cdc42
Q8N392ARHGAP18Rho GTPase-activating protein 18Rac, RhoQ, RhoU
O43182ARHGAP6Rho GTPase-activating protein 6Rac
P11274BCRBreakpoint cluster region proteinRac, Cdc42
Q96P48ARAP1Arf-GAP with Rho-GAP, ANK repeat, PH domains 1?
Q9P107GMIPGEM-interacting proteinRhoA
P98171ARHGAP4Rho GTPase-activating protein 4Rac
Q92502STARD8StAR-related lipid transfer protein 8RhoA, Cdc42
Q07960ARHGAP1Rho GTPase-activating protein 1Cdc42
Q7Z6I6ARHGAP30Rho GTPase-activating protein 30Rac, RhoH
Q9BRR9ARHGAP9Rho GTPase-activating protein 9Cdc42, Rac, RhoA
O60890OPHN1Oligophrenin-1RhoA, Rac
A1A4S6ARHGAP10Rho GTPase-activating protein 10RhoA, Cdc42
P42331ARHGAP25Rho GTPase-activating protein 25?
Q12979ABRActive breakpoint cluster region-related proteinRac, Cdc42
Q6ZUM4ARHGAP27Rho GTPase-activating protein 27Cdc42, Rac
Q8N103TAGAPT-cell activation Rho GTPase-activating protein?
P52566ARHGDIBRho GDP-dissociation inhibitor 2Rac, RhoA, RhoB, RhoH
P52565ARHGDIARho GDP-dissociation inhibitor 1Rac, RhoA, RhoH