Table 1 PCR primers used for the gene cloning
GenePrimer-1Primer-2
ilvD5′-atagagctcatgcctaagtaccgttccgccacc-3′5′-cgcgaattcttaaccccccagtttcgatttatc-3′
thiC5′-ataccatggctgcaacaaaactgacccg-3′5′-tctaagcttcgcttcctccttacgcaggtag-3′
acnB5′-gaaccgccatggtagaagaatacc-3′5′-tgactttttaaagcttagtctgga-3′
nth5′-aatgtcccatggataaagcaaaac-3′5′-ttcttcaaagcttaactttctctt-3′
fdx5′-aggtttaccatggcaaagattgtt-3′5′-cctctgttaaagcttacgcgcatg-3′
soxR5′-atagagctcatggaaaagaaattacccc-3′5′-cgcgaattcttagttttgttcatcttcc-3′
fhuF5′-tatccatggcctatcgttccgcaccgctctatg-3′5′-gcgaagctttttcagcgtacaatcgccacattg-3′