Table 3 Kinetic parameters for selective COX2 inhibition by the B2 dimer and C1 trimer

Initial reaction rates were determined as described in Table 2. Data were fitted to the standard Michaelis–Menten kinetic equation. Results are represented as the means±S.E.M. for three independent experiments, and the significance of the differences between inhibitors for each kinetic parameter compared with the control condition was analysed by ANOVA (*P<0.05).

SampleVmax (μM/min)Km (μM)kcat (s−1)kcat/Km (s−1·μM−1)Ki (μM)
Control(0.198±0.077)×10−34.22±0.48(4.11±0.16)×10−3(0.972±0.012)×10−3
B2(0.252±0.001)×10−39.71±1.00*(5.25±0.22)×10−3(0.54±0.00155)×10−3*7.75±0.68
C1(0.177±0.015)×10−36.78±1.01*(3.69±0.21)×10−3(0.467±0.0423)×10−3*11.69±1.25