Table 2 Kinetic parameters for selective COX1 inhibition by the DHA and B1 dimers

Initial reaction rates were determined using a cell-free assay for various concentrations of AA (0.15–25 μM). Data were fitted to the standard Michaelis–Menten kinetic equation and Lineweaver–Burk transformation. The results are represented as means±S.E.M. for three independent experiments, and the significance of the differences between the inhibitors for each kinetic parameter compared with the control condition was analysed by ANOVA (*P<0.05).

SampleVmax (μM/min)Km (μM)kcat (s−1)kcat/Km (s−1·μM−1)Ki (μM)
Control(2.14±0.016)×10−3(33±3.5)×10−3(0.151±1.0)×10−34.63±0.47
DHA(1.34±0.05)×10−3*(762±11)×10−3*(0.094±4.1)×10−30.13±0.03*0.73±0.17
B1(1.95±0.026)×10−3(19±9.4)×10−3*(0.137±2.5)×10−31.32±0.10*6.87±0.66