Table 2 Kinetic parameters for selective COX1 inhibition by the DHA and B1 dimers

Initial reaction rates were determined using a cell-free assay for various concentrations of AA (0.15–25 μM). Data were fitted to the standard Michaelis–Menten kinetic equation and Lineweaver–Burk transformation. The results are represented as means±S.E.M. for three independent experiments, and the significance of the differences between the inhibitors for each kinetic parameter compared with the control condition was analysed by ANOVA (*P<0.05).

SampleVmax (μM/min)Km (μM)kcat (s−1)kcat/Km (s−1·μM−1)Ki (μM)