Table 1 Properties of PLCζ-luciferase and mutants expressed in mouse eggs

Ca2+ oscillation-inducing activity (Ca2+ spike number in 2 h; time to first Ca2+ spike) and luciferase luminescence levels (peak luminescence; luminescence at first spike) are summarized for mouse eggs microinjected with each PLC-luciferase construct (see Figure 1B). Each egg was microinjected with a pipette cRNA concentration of 1.6 g/l. Values are means±S.E.M.

PLCζ-luciferaseCa2+ spikes in the first 2 h (n)Peak luminescence (c.p.s.)Time of first spike (min)Luminescence at first spike (c.p.s.)Number of eggs
PLCζWT19±0.147.33±0.38~300.35±0.03717
PLCζH435P010.7±0.6217
PLCζH435A10±0.651.74±0.14~550.73±0.03822
PLCζD210R08.6±0.5522