Table 4 ESI–TOF (time-of-flight) MS analyses of the native and mutant RrgB proteins
Maverage* (Da)
RrgBExpectedObserved by ESI–TOFΔ(MexpectedMobserved) (Da)NH3 units lost
Wild-type6745367399543
K41A6734367396523
E143A6739567341543
N184A6735767410523
  • * Average molecular mass.

  • Difference between expected and observed molecular mass.