Table 4 Specific enzyme activities of Lm-thiolase and Tb-thiolase

1 unit is defined as 1 μmol of conversions per min. ND, not detectable.

ProteinConstructThiolytic direction (units/mg)Synthetic direction (units/mg)
Lm-thiolaseWild-type2.57±0.0118.04±1.93
C123ANDND
C123S0.41±0.07ND
Tb-thiolaseWild-type2.87±0.2318.53±1.27
C120ANDND
C337ANDND