Table 2 Crystallization and crystal handling

PEGMME, poly(ethylene glycol) monomethyl ether.

ProteinConstructCrystallization conditionsCryoprotectant buffer
Lm-thiolaseWild-type100 mM Mes, pH 6.5, 10% PEGMME 5000, 10% DMSO and 10% MPD100 mM Mes, pH 6.5, 10% PEGMME 5000, 10% DMSO and 15% MPD
C123A and CoA100 mM Tris/HCl, pH 8.0, 200 mM NaCl, 20% PEG 4000 and 1 mM CoA100 mM Tris/HCl, pH 8.0, 200 mM NaCl, 30% PEG 4000 and 1 mM CoA
C123A100 mM Mes, pH 6.5, 10% PEGMME 5000, 10% DMSO and 10% MPD100 mM Mes, pH 6.5, 10% PEGMME 5000, 10% DMSO and 15% MPD
C123S100 mM Mes, pH 6.5, 10% PEGMME 5000, 10% DMSO and 10% MPD100 mM Mes, pH 6.5, 10% PEGMME 5000, 10% DMSO and 15% MPD
Tb-thiolaseWild-type100 mM sodium citrate, pH 5.6, 200 mM ammonium acetate and 30% PEG 4000Mounted directly from the drop
C337A100 mM Bis-Tris, pH 5.5, 200 mM lithium sulfate and 25% PEG 3350Mounted directly from the drop