Table 5 Relative potencies of small-molecule kinase inhibitors towards different protein kinases

IC50 values were determined from assays carried out at ten different inhibitor concentrations.

CompoundProtein kinaseIC50 (μM)ATP in assay (μM)
FMKSrc0.5650
Lck0.7550
S6K10.9520
Yes0.5120
Eph-A20.7650
WortmanninsmMLCK5.050
PLK11.35
LY 294002PLKI2.05
PIM10.8220
PIM31.420
D4476CK10.3100
PKD19.150
p38αMAPK5.850
HarmineDYRK1A0.0850
DYRK20.950
DYRK30.850
PIM34.320
CK11.520
RoscovitineCDK20.1420
PAK46.95
PurvalanolCDK20.0320
PAK40.135
VX 680Aurora B0.04100
Aurora C0.07100
Src0.3450
MELK0.4250
SU 6668Aurora B0.03520
Aurora C0.215
STO 609CaMKKβ0.0120
CaMKKα0.1220
MNKI0.1250
CK20.195
AMPK0.1650
PIM20.115
PIM30.083100
DYRK20.9550
PS 1145IKKβ0.255
PIMI1.120
PIM30.8820
BMS 345541IKKβ2.65
SC 514IKKβ2.05
AS 601245JNK12.620
JNK25.020
GSK30.0420
PIM10.0820
PIM30.035
DYRK20.350
CGP 57380MNKI0.8750
MNK21.650
CKI0.5120
Aurora B2.520
DYRK33.25
SGK12.720
BRSK21.150
Lck2.550