In the present paper we describe a new carboxylic acid transporter in Escherichia coli encoded by the gene yaaH. In contrast to what had been described for other YaaH family members, the E. coli transporter is highly specific for acetic acid (a monocarboxylate) and for succinic acid (a dicarboxylate), with affinity constants at pH 6.0 of 1.24±0.13 mM for acetic acid and 1.18±0.10 mM for succinic acid. In glucose-grown cells the ΔyaaH mutant is compromised for the uptake of both labelled acetic and succinic acids. YaaH, together with ActP, described previously as an acetate transporter, affect the use of acetic acid as sole carbon and energy source. Both genes have to be deleted simultaneously to abolish acetate transport. The uptake of acetate and succinate was restored when yaaH was expressed in trans in ΔyaaH ΔactP cells. We also demonstrate the critical role of YaaH amino acid residues Leu131 and Ala164 on the enhanced ability to transport lactate. Owing to its functional role in acetate and succinate uptake we propose its assignment as SatP: the Succinate–Acetate Transporter Protein.
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Research Article|
August 29 2013
SATP (YaaH), a succinate–acetate transporter protein in Escherichia coli
Joana Sá-Pessoa;
Joana Sá-Pessoa
*Centre of Molecular and Environmental Biology (CBMA), Department of Biology, University of Minho, Campus de Gualtar, Braga, 4710-057, Portugal
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Sandra Paiva;
Sandra Paiva
*Centre of Molecular and Environmental Biology (CBMA), Department of Biology, University of Minho, Campus de Gualtar, Braga, 4710-057, Portugal
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David Ribas;
David Ribas
*Centre of Molecular and Environmental Biology (CBMA), Department of Biology, University of Minho, Campus de Gualtar, Braga, 4710-057, Portugal
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Inês Jesus Silva;
Inês Jesus Silva
†Instituto de Tecnologia Química e Biológica (ITQB), Universidade Nova de Lisboa, Av. da República, 2780-157 Oeiras, Portugal
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Sandra Cristina Viegas;
Sandra Cristina Viegas
†Instituto de Tecnologia Química e Biológica (ITQB), Universidade Nova de Lisboa, Av. da República, 2780-157 Oeiras, Portugal
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Cecília Maria Arraiano;
Cecília Maria Arraiano
†Instituto de Tecnologia Química e Biológica (ITQB), Universidade Nova de Lisboa, Av. da República, 2780-157 Oeiras, Portugal
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Margarida Casal
Margarida Casal
1
*Centre of Molecular and Environmental Biology (CBMA), Department of Biology, University of Minho, Campus de Gualtar, Braga, 4710-057, Portugal
1To whom correspondence should be addressed (email mcasal@bio.uminho.pt).
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Publisher: Portland Press Ltd
Received:
March 20 2013
Revision Received:
July 03 2013
Accepted:
July 11 2013
Accepted Manuscript online:
July 11 2013
Online ISSN: 1470-8728
Print ISSN: 0264-6021
© The Authors Journal compilation © 2013 Biochemical Society
2013
Biochem J (2013) 454 (3): 585–595.
Article history
Received:
March 20 2013
Revision Received:
July 03 2013
Accepted:
July 11 2013
Accepted Manuscript online:
July 11 2013
Citation
Joana Sá-Pessoa, Sandra Paiva, David Ribas, Inês Jesus Silva, Sandra Cristina Viegas, Cecília Maria Arraiano, Margarida Casal; SATP (YaaH), a succinate–acetate transporter protein in Escherichia coli. Biochem J 15 September 2013; 454 (3): 585–595. doi: https://doi.org/10.1042/BJ20130412
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