In order to redefine the mannitol pathway in the necrotrophic plant pathogen Botrytis cinerea, we used a targeted deletion strategy of genes encoding two proteins of mannitol metabolism, BcMTDH (B. cinerea mannitol dehydrogenase) and BcMPD (B. cinerea mannitol-1-phosphate dehydrogenase). Mobilization of mannitol and quantification of Bcmpd and Bcmtdh gene transcripts during development and osmotic stress confirmed a role for mannitol as a temporary and disposable carbon storage compound. In order to study metabolic fluxes, we followed conversion of labelled hexoses in wild-type and ΔBcmpd and ΔBcmtdh mutant strains by in vivo NMR spectroscopy. Our results revealed that glucose and fructose were metabolized via the BcMPD and BcMTDH pathways respectively. The existence of a novel mannitol phosphorylation pathway was also suggested by the NMR investigations. This last finding definitively challenged the existence of the originally postulated mannitol cycle in favour of two simultaneously expressed pathways. Finally, physiological and biochemical studies conducted on double deletion mutants (ΔBcmpdΔBcmtdh) showed that mannitol was still produced despite a complete alteration of both mannitol biosynthesis pathways. This strongly suggests that one or several additional undescribed pathways could participate in mannitol metabolism in B. cinerea.
Skip Nav Destination
Article navigation
April 2010
-
Cover Image
Cover Image
- PDF Icon PDF LinkFront Matter
- PDF Icon PDF LinkTable of Contents
- PDF Icon PDF LinkEditorial Board
Research Article|
March 29 2010
Novel insights into mannitol metabolism in the fungal plant pathogen Botrytis cinerea
Thierry Dulermo;
Thierry Dulermo
*Génomique Fonctionnelle des Champignons Pathogènes des Plantes, Unité Mixte de Recherche 5240 Microbiologie, Adaptation et Pathogénie, Université de Lyon, Lyon F-69003, France; Université Lyon1-Centre National de la Recherche Scientifique-Institut National des Sciences Appliquées-BayerCropScience, 10 rue Pierre Baizet, Lyon F-69009, France
†Microbiologie & Génétique Moléculaire, Centre National de la Recherche Scientifique, Unité Mixte de Recherche 2585; Institut National de la Recherche Agronomique, Unité mixte de Recherche 1238, AgroParisTech, centre de Versailles-Grignon BP 01, Thiverval-Grignon F-78850, France
Search for other works by this author on:
Christine Rascle;
Christine Rascle
*Génomique Fonctionnelle des Champignons Pathogènes des Plantes, Unité Mixte de Recherche 5240 Microbiologie, Adaptation et Pathogénie, Université de Lyon, Lyon F-69003, France; Université Lyon1-Centre National de la Recherche Scientifique-Institut National des Sciences Appliquées-BayerCropScience, 10 rue Pierre Baizet, Lyon F-69009, France
Search for other works by this author on:
Geneviève Billon-Grand;
Geneviève Billon-Grand
*Génomique Fonctionnelle des Champignons Pathogènes des Plantes, Unité Mixte de Recherche 5240 Microbiologie, Adaptation et Pathogénie, Université de Lyon, Lyon F-69003, France; Université Lyon1-Centre National de la Recherche Scientifique-Institut National des Sciences Appliquées-BayerCropScience, 10 rue Pierre Baizet, Lyon F-69009, France
Search for other works by this author on:
Elisabeth Gout;
Elisabeth Gout
‡Physiologie Cellulaire & Végétale, Unité Mixte de Recherche 5168, Institut de Recherche en Technologies et Sciences pour le Vivant, Commissariat à l'Energie Atomique, 17 rue des Martyrs, 38054 Grenoble cedex 9, France
Search for other works by this author on:
Richard Bligny;
Richard Bligny
‡Physiologie Cellulaire & Végétale, Unité Mixte de Recherche 5168, Institut de Recherche en Technologies et Sciences pour le Vivant, Commissariat à l'Energie Atomique, 17 rue des Martyrs, 38054 Grenoble cedex 9, France
Search for other works by this author on:
Pascale Cotton
Pascale Cotton
1
§Génétique Moléculaire des Levures, Unité Mixte de Recherche 5240, Microbiologie, Adaptation et Pathogénie, Université de Lyon, Lyon, F-69003, France; Université Lyon1-Centre National de la Recherche Scientifique-Institut National des Sciences Appliquées-BayerCropScience, 10 rue Raphaël Dubois, Bât Lwoff, Villeurbanne F-69621, France
1To whom correspondence should be addressed (email pascale.cotton@univ-lyon1.fr).
Search for other works by this author on:
Publisher: Portland Press Ltd
Received:
November 25 2009
Revision Received:
February 03 2010
Accepted:
February 05 2010
Accepted Manuscript online:
February 05 2010
Online ISSN: 1470-8728
Print ISSN: 0264-6021
© The Authors Journal compilation © 2010 Biochemical Society
2010
Biochem J (2010) 427 (2): 323–332.
Article history
Received:
November 25 2009
Revision Received:
February 03 2010
Accepted:
February 05 2010
Accepted Manuscript online:
February 05 2010
Citation
Thierry Dulermo, Christine Rascle, Geneviève Billon-Grand, Elisabeth Gout, Richard Bligny, Pascale Cotton; Novel insights into mannitol metabolism in the fungal plant pathogen Botrytis cinerea. Biochem J 15 April 2010; 427 (2): 323–332. doi: https://doi.org/10.1042/BJ20091813
Download citation file:
Sign in
Don't already have an account? Register
Sign in to your personal account
You could not be signed in. Please check your email address / username and password and try again.
Could not validate captcha. Please try again.