Human fibrinogen is an important coagulation factor as well as an independent predictor of coronary heart disease and stroke. Analysis of dysfibrinogens may provide useful information and help us to understanding the molecular defects in fibrin polymerization. Here the influence of oxidative stress of fibrinogen induced by H2O2 to the polymerization state of fibrin was studied. UV absorbance spectroscopy, circular dichroism, zeta-potential, dynamic light scattering, steady shear viscosity were all employed to s tudy the influence of oxidative stress to the molecular structure, the surface charges, the size and shape of fibrinogen molecules. The obtained fibrin morphology was imaged and investigated with atomic force microscope. The results demonstrated that the cross-linking, the branching and the height distribution of formed fibrin will be influenced by the oxidative stress of fibrinogen. This work presented a new insight into the aggregation behaviour of fibrinogen and will be helpful to understand the formation mechanism of thrombosis under oxidative stress.
- oxidative stress
- aggregation state
- ©2016 The Author(s)
This is an Accepted Manuscript; not the final Version of Record. Archiving permitted only in line with the archiving policy of Portland Press Limited. All other rights reserved.