MiR-592 has been identified as a neural-enriched microRNA, plays an important role in mNPCs differentiation, could induce astrogliogenesis differentiation arrest or/and enhance neurogenesis in vitro . Previous studies showed that long non-coding RNAs (lncRNAs) were involved in the neuronal development and activity. In order to investigate the role of miR-592 in neurogenesis, we described the expression profile of lncRNAs in miR-592 knock-out mouse embryonic stem cells (mESCs) and the corresponding normal mESCs by microarray. By the microarray analysis and luciferase reporter assays, we demonstrated that lncRNA - AK048794, regulated by transcription factor GATA1, functioned as a competing endogenous RNA (ceRNA) for miR-592 and led to the de-repression of its endogenous target FAM91A1, which involved in mESCs pluripotency maintenance. Taken together, these observations imply that AK048794 modulated the expression of multiple genes involved in mESCs pluripotency maintenance by acting as a competing endogenous RNA for miR-592, which may build up the link between the regulatory miRNA network and mESCs pluripotency.
- ©2016 The Author(s)
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