Structural mechanisms behind variations in glycosaminoglycan (GAG) affinities of decorin-binding protein As (DBPAs) from different Borrelia strains were investigated using NMR. DBPA from strain PBr was revealed to have an additional GAG-binding epitope and a retracted linker allowing more access to its GAG-binding sites.
- glycosaminoglycan-binding protein
- Lyme disease
- nuclear magnetic resonance (NMR)
Abbreviations: DBPA, decorin-binding protein A; dp6, degree of polymerization 6; DS, dermatan sulfate; EDC, 1-ethyl-3-(3-dimethyl-aminopropyl)carbodiimide; GAG, glycosaminoglycan; GMSA, gel mobility-shift assay; HRP, horseradish peroxidase; ORF, open reading frame; RDCs, residual dipolar couplings; PRE, paramagnetic relaxation enhancement; TEMPO, (2,2,6,6-tetramethylpiperidin-1-yl)oxyl; TMB, tetramethylbenzidine; WT, wild-type
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