Polyclonal autoantibodies against human GM-CSF (granulocyte/macrophage colony-stimulating factor) are a hallmark of PAP (pulmonary alveolar proteinosis) and several other reported autoimmune diseases. MB007 is a high-affinity anti-(human GM-CSF) autoantibody isolated from a patient suffering from PAP which shows only modest neutralization of GM-CSF bioactivity. We describe the first crystal structure of a cytokine-directed human IgG1λ autoantibody-binding fragment (Fab) at 1.9 Å (1 Å=0.1 nm) resolution. Its CDR3-H substantially differs from all VH7 germline IgG1 structures reported previously. We derive a reliable model of the antigen–autoantibody complex by using NMR chemical shift perturbation data in combination with computational methods. Superposition of the modelled complex structure with the human GM-CSF–GM-CSF ternary receptor complex reveals only little overlap between receptor and Fab when bound to GM-CSF. Our model provides a structural basis for understanding the mode of action of the MB007 autoantibody.
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Research Article|
September 26 2012
Molecular structure of human GM-CSF in complex with a disease-associated anti-human GM-CSF autoantibody and its potential biological implications
Michaela Blech;
Michaela Blech
1
*Department of Lead Identification and Optimization Support, Structural Research Group, Boehringer Ingelheim Pharma GmbH & Co. KG, Birkendorfer Strasse 65, 88397 Biberach, Germany
†Department of NBE Discovery, Boehringer Ingelheim Pharma GmbH & Co. KG, Birkendorfer Strasse 65, 88397 Biberach, Germany
‡Institut für Medizintechnologie der Ruprecht-Karls-Universität Heidelberg & Hochschule Mannheim, Heidelberg and Mannheim, Germany
1Correspondence may be addressed to either of these authors (email michaela.blech@boehringer-ingelheim.com or john.park@boehringer-ingelheim.com).
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Daniel Seeliger;
Daniel Seeliger
§Department of Lead Identification and Optimization Support, Computational Chemistry Group, Boehringer Ingelheim Pharma GmbH & Co. KG, Birkendorfer Strasse 65, 88397 Biberach, Germany
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Barbara Kistler;
Barbara Kistler
∥Department of Respiratory Diseases Research, Boehringer Ingelheim Pharma GmbH & Co. KG, Birkendorfer Strasse 65, 88397 Biberach, Germany
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Margit M. T. Bauer;
Margit M. T. Bauer
*Department of Lead Identification and Optimization Support, Structural Research Group, Boehringer Ingelheim Pharma GmbH & Co. KG, Birkendorfer Strasse 65, 88397 Biberach, Germany
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Mathias Hafner;
Mathias Hafner
‡Institut für Medizintechnologie der Ruprecht-Karls-Universität Heidelberg & Hochschule Mannheim, Heidelberg and Mannheim, Germany
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Stefan Hörer;
Stefan Hörer
*Department of Lead Identification and Optimization Support, Structural Research Group, Boehringer Ingelheim Pharma GmbH & Co. KG, Birkendorfer Strasse 65, 88397 Biberach, Germany
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Markus Zeeb;
Markus Zeeb
*Department of Lead Identification and Optimization Support, Structural Research Group, Boehringer Ingelheim Pharma GmbH & Co. KG, Birkendorfer Strasse 65, 88397 Biberach, Germany
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Herbert Nar;
Herbert Nar
*Department of Lead Identification and Optimization Support, Structural Research Group, Boehringer Ingelheim Pharma GmbH & Co. KG, Birkendorfer Strasse 65, 88397 Biberach, Germany
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John E. Park
John E. Park
1
†Department of NBE Discovery, Boehringer Ingelheim Pharma GmbH & Co. KG, Birkendorfer Strasse 65, 88397 Biberach, Germany
1Correspondence may be addressed to either of these authors (email michaela.blech@boehringer-ingelheim.com or john.park@boehringer-ingelheim.com).
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Publisher: Portland Press Ltd
Received:
May 30 2012
Revision Received:
July 10 2012
Accepted:
July 27 2012
Accepted Manuscript online:
July 27 2012
Online ISSN: 1470-8728
Print ISSN: 0264-6021
© The Authors Journal compilation © 2012 Biochemical Society
2012
Biochem J (2012) 447 (2): 205–215.
Article history
Received:
May 30 2012
Revision Received:
July 10 2012
Accepted:
July 27 2012
Accepted Manuscript online:
July 27 2012
Citation
Michaela Blech, Daniel Seeliger, Barbara Kistler, Margit M. T. Bauer, Mathias Hafner, Stefan Hörer, Markus Zeeb, Herbert Nar, John E. Park; Molecular structure of human GM-CSF in complex with a disease-associated anti-human GM-CSF autoantibody and its potential biological implications. Biochem J 15 October 2012; 447 (2): 205–215. doi: https://doi.org/10.1042/BJ20120884
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