The genetic element pSSVx from Sulfolobus islandicus, strain REY15/4, is a hybrid between a plasmid and a fusellovirus. This plasmid–virus hybrid infects several species of the hyperthermophilic acidophilic crenarchaeon Sulfolobus. The open reading frame orfc68 of pSSVx encodes a 7.7 kDa protein that does not show significant sequence homology with any protein with known three-dimensional structure. EMSA (electrophoretic mobility-shift assay) experiments, DNA footprinting and CD analyses indicate that recombinant C68, purified from Escherichia coli, binds to two different operator sites that are located upstream of its own promoter. The three-dimensional structure, solved by a single-wavelength anomalous diffraction experiment on a selenomethionine derivative, shows that the protein assumes a swapped-hairpin fold, which is a distinctive fold associated with a family of prokaryotic transcription factors, such as AbrB from Bacillus subtilis. Nevertheless, C68 constitutes a novel representative of this family because it shows several peculiar structural and functional features.
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Research Article|
March 15 2011
C68 from the Sulfolobus islandicus plasmid–virus pSSVx is a novel member of the AbrB-like transcription factor family
Patrizia Contursi;
*Dipartimento di Biologia Strutturale e Funzionale, Università degli Studi di Napoli Federico II, Complesso Universitario Monte S. Angelo, Via Cinthia, Naples, Italy
‡Archaeal Genetics Laboratory, Department of Biology, University of Copenhagen, Ole Maaløes Vej 5, DK-2200 Copenhagen N, Denmark
2Correspondence may be addressed to either of these authors (email contursi@unina.it or empedone@unina.it).
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Katia D'Ambrosio;
Katia D'Ambrosio
1
†Istituto di Biostrutture e Bioimmagini-CNR, via Mezzocannone 16, 80134 Naples, Italy
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Luciano Pirone;
Luciano Pirone
†Istituto di Biostrutture e Bioimmagini-CNR, via Mezzocannone 16, 80134 Naples, Italy
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Emilia Pedone;
Emilia Pedone
2
†Istituto di Biostrutture e Bioimmagini-CNR, via Mezzocannone 16, 80134 Naples, Italy
2Correspondence may be addressed to either of these authors (email contursi@unina.it or empedone@unina.it).
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Tiziana Aucelli;
Tiziana Aucelli
*Dipartimento di Biologia Strutturale e Funzionale, Università degli Studi di Napoli Federico II, Complesso Universitario Monte S. Angelo, Via Cinthia, Naples, Italy
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Qunxin She;
Qunxin She
‡Archaeal Genetics Laboratory, Department of Biology, University of Copenhagen, Ole Maaløes Vej 5, DK-2200 Copenhagen N, Denmark
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Giuseppina De Simone;
Giuseppina De Simone
†Istituto di Biostrutture e Bioimmagini-CNR, via Mezzocannone 16, 80134 Naples, Italy
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Simonetta Bartolucci
Simonetta Bartolucci
*Dipartimento di Biologia Strutturale e Funzionale, Università degli Studi di Napoli Federico II, Complesso Universitario Monte S. Angelo, Via Cinthia, Naples, Italy
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Publisher: Portland Press Ltd
Received:
August 25 2010
Revision Received:
December 21 2010
Accepted:
January 05 2011
Accepted Manuscript online:
January 05 2011
Online ISSN: 1470-8728
Print ISSN: 0264-6021
© The Authors Journal compilation © 2011 Biochemical Society
2011
Biochem J (2011) 435 (1): 157–166.
Article history
Received:
August 25 2010
Revision Received:
December 21 2010
Accepted:
January 05 2011
Accepted Manuscript online:
January 05 2011
Citation
Patrizia Contursi, Katia D'Ambrosio, Luciano Pirone, Emilia Pedone, Tiziana Aucelli, Qunxin She, Giuseppina De Simone, Simonetta Bartolucci; C68 from the Sulfolobus islandicus plasmid–virus pSSVx is a novel member of the AbrB-like transcription factor family. Biochem J 1 April 2011; 435 (1): 157–166. doi: https://doi.org/10.1042/BJ20101334
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