Biochemical Journal

Review article

AMPK and the biochemistry of exercise: implications for human health and disease

Erik A. Richter, Neil B. Ruderman


AMPK (AMP-activated protein kinase) is a phylogenetically conserved fuel-sensing enzyme that is present in all mammalian cells. During exercise, it is activated in skeletal muscle in humans, and at least in rodents, also in adipose tissue, liver and perhaps other organs by events that increase the AMP/ATP ratio. When activated, AMPK stimulates energy-generating processes such as glucose uptake and fatty acid oxidation and decreases energy-consuming processes such as protein and lipid synthesis. Exercise is perhaps the most powerful physiological activator of AMPK and a unique model for studying its many physiological roles. In addition, it improves the metabolic status of rodents with a metabolic syndrome phenotype, as does treatment with AMPK-activating agents; it is therefore tempting to attribute the therapeutic benefits of regular physical activity to activation of AMPK. Here we review the acute and chronic effects of exercise on AMPK activity in skeletal muscle and other tissues. We also discuss the potential role of AMPK activation in mediating the prevention and treatment by exercise of specific disorders associated with the metabolic syndrome, including Type 2 diabetes and Alzheimer's disease.

  • AMP-activated protein kinase (AMPK)
  • exercise
  • muscle
  • metabolic syndrome


AMPK (AMP-activated protein kinase) is a phylogenetically conserved fuel-sensing enzyme that is present in both primitive unicellular organisms and mammals [1]. It is activated by stresses that increase the cellular concentration of AMP relative to ATP due to either limited ATP production (e.g. glucose deprivation or hypoxia) or increased energy expenditure (e.g. muscle contraction). When this occurs, AMPK sets in motion processes that potentially both increase ATP generation such as fatty acid oxidation and glucose transport, and decreases others that consume ATP, but are not acutely required for survival, such as lipid and protein synthesis and cell growth and proliferation [1,2]. In addition, it may specifically stimulate glycolysis in cardiac muscle [3]. Recent evidence suggests that AMPK may have a much wider range of actions. For instance, it is involved in the regulation of such diverse events as mitochondrial biogenesis [4,5], angiogenesis [6], cell polarity [7] and the control of food intake and whole-body energy expenditure at the level of the hypothalamus [8]. In addition, AMPK activation in peripheral tissues seems to counteract many of the cellular abnormalities observed in animal models of the metabolic syndrome, including insulin resistance, inflammation and ectopic lipid deposition [913]. Conversely, its dysregulation (defined as decreased activity or impaired activation) may contribute to these abnormalities [14].

Exercise, which is perhaps the most extreme metabolic stress experienced by normal humans, leads to activation of AMPK in skeletal muscle [1517] and, at least in rodents in intra-abdominal adipose tissue, liver [18,19] and probably other organs (J. Cacicedo, M.-S. Gauthier and N. B. Ruderman, unpublished work). It also has effects on insulin-sensitivity [2022] and gene and protein expression in various tissues [19,2325], and in humans, it reduces overall morbidity and mortality in an otherwise sedentary population [26]. In contrast, physical inactivity is known to be a powerful risk factor for many diseases and is beginning to be considered as a disease by itself [27]. It can be hypothesized that many of the beneficial effects of exercise and the adverse effects of physical inactivity are related, respectively, to the activation or lack of activation of AMPK. From a biochemical perspective, exercise can be used both as a model to study the mechanisms by which AMPK is activated in skeletal muscle and other tissues and as a tool to unravel its physiological roles in vivo. In the present review, we examine these possibilities, with special emphasis on the apparent ability of exercise to prevent and treat various diseases.


AMPK is an heterotrimeric enzyme composed of a catalytic α-subunit and regulatory β- and γ-subunits. The α- and β-subunits each exist in two isoforms (α1 and α2, and β1 and β2), and the γ-subunit in three isoforms (γ1, γ2 and γ3). The γ-subunit contains two pairs of Bateman [CBS (cystathionine β-synthase)] domains that bind AMP and ATP. According to recent findings, on the basis of the crystal structure of the γ-subunit, it has been suggested that two sites bind either AMP or ATP, whereas a third site contains a tightly bound AMP that does not exchange [28]. Furthermore, under conditions in which the cell's energy state is not depressed, it is believed that ATP is predominantly bound to the two Bateman domains and that most AMPK molecules are inactive [28].

Physiological activation of AMPK occurs in skeletal muscle during exercise in response to increased binding of AMP and decreased binding of ATP to the γ-subunit (Figure 1). Exercise can be characterized by a large (>100-fold) increase in muscle energy turnover and by alterations in nucleotide status. Although ADP is the direct product of the hydrolysis of ATP during muscle contraction, it is converted rapidly into AMP via the adenylate kinase reaction (Figure 2). This in turn leads to large increases in AMP concentration dependent on the intensity and duration of the exercise, even though free cytosolic AMP is buffered by protein binding and deamination to IMP [29]. In contrast, ATP concentrations change little during exercise, unless the exercise is very intense. AMP activates AMPK, and ATP antagonizes this effect. Therefore, as already noted, it is the ratio between AMP and ATP that is of importance for AMPK activation [30]. AMP binding to the Bateman domains can stimulate AMPK allosterically, but apparently this only has a moderate activating effect (<10-fold) [31]. More importantly, AMP binding leads to increased AMPK phosphorylation at Thr172 of the α-subunit which can enhance AMPK activity more than 100-fold [32]. The importance of an increase in the AMP/ATP ratio in mediating AMPK phosphorylation during exercise is underscored by the fact that it is impaired in adenylate kinase-deficient mice in which AMP generation is decreased during muscle contractions [33]. The mechanisms by which AMP activates AMPK have been challenged recently. Previous observations suggested that AMP binding to the Bateman domains of the γ-subunit of AMPK, in addition to allosterically activating AMPK, make it a much better substrate for upstream AMPK kinases and a poorer substrate for phosphatases [30]. More recent studies, however, suggest that the major effect of AMP binding to AMPK is to inhibit the action of phosphatases [34,35]. According to this scheme, the major upstream kinase of AMPK, LKB1, is constitutively active in muscle and, in the basal state, AMPK is continuously phosphorylated and dephosphorylated in a futile cycle. Although this may seem energetically wasteful, the energy cost of this cycling is quantitatively negligible and it allows for greater and more rapid changes in the phosphorylation status and activity of AMPK in response to various stimuli.

Figure 1 Activation of AMPK in skeletal muscle during contraction/exercise.

Although LKB1, the main kinase acting on α2-AMPK, is thought to be constitutively active, the possibility exists that SIRT1 may regulate LKB1 activity perhaps during prolonged exercise. In muscle, CaMKK may act as an AMPK kinase, especially towards α1-AMPK under conditions in which AMP accumulation is limited. The role of TAK1 in muscle is uncertain. Binding of AMP to the γ-subunit of AMPK makes AMPK a poor substrate for PP2C (protein phosphatase 2C), resulting in increased net phosphorylation of AMPK. IL-6 produced by contracting muscle may increase AMPK activity in this or adjacent muscle by an as yet unknown mechanism (broken line). The β-subunit of AMPK has a glycogen-binding domain, and high glycogen levels in muscle inhibit AMPK activation during exercise. T172-P, phosphorylated Thr172.

Figure 2 During exercise, ATP is degraded to ADP

ADP in turn is in part reconverted into ATP and AMP via the adenylate kinase reaction. The AMP/ATP ratio is very sensitive to changes in high energy phosphates because the adenylate kinase reaction is in equilibrium. Thus [ATP][AMP]/[ADP]2=K and [ATP][AMP] =K×[ADP]2. If both sides of the latter equation are divided by [ATP]2, we get [AMP]/[ATP] ≈([ADP]/[ATP])2, indicating that the AMP/ATP ratio varies approximately as the square of the ADP/ATP ratio.


LKB1 has been identified as an important upstream AMPK kinase in muscle and most other cells [36,37]. Its importance during electrically induced muscle contractions has been demonstrated by the severely blunted activation of α2-AMPK and ACCβ (acetyl-CoA carboxylase β) phosphorylation in muscle-specific LKB1-KO (knockout) mice [38,39]. Interestingly, α1-AMPK activation was less affected [38,39]. In addition, exercise capacity in LKB1-KO mice is markedly impaired compared with WT (wild-type) mice [40]. Although there is ample evidence for the importance of LKB1 for AMPK activation in muscle, the activity of LKB1 does not appear to be increased in muscle during exercise [38,41], supporting the notion that it is constitutively active.

CaMKK [CaMK (Ca2+/calmodulin-dependent protein kinase) kinase], TAK1 [TGF (transforming growth factor)-β-activated kinase 1] and SIRT1 (silent information regulator 1)

Although LKB1 is the predominant AMPK kinase in most cells, a Ca2+-dependent CaMKKβ has been found to phosphorylate AMPK at Thr172 in brain, endothelium and lymphocytes [42,43]. Recently, it has been reported that CaMKK may also act as an upstream AMPK kinase early on during contractions in skeletal muscle [44] and that CaMKKα may be the important isoform in muscle [45], in contrast with other tissues in which CaMKKβ seems to be the dominant isoform [42,43]. Also of note, when the intracellular Ca2+-concentration was increased in skeletal muscle by incubation with caffeine, it resulted in increased AMPK activity, due primarily to activation and phosphorylation of α1-AMPK [46]. Although definitive genetic studies are lacking, collectively these observation suggest that CaMKK in fact acts as an upstream AMPK kinase in skeletal muscle, perhaps mainly for α1-AMPK. They also suggest that an intensity- and/or time-dependent switch may occur in the relative importance of AMPK kinases during contraction.

Another potentially relevant enzyme that has been studied in cultured cardiomyocytes is the mitogen-activated TAK1. It has been suggested that TAK1 is either a LKB1-regulating kinase or a functional AMPK kinase [47]. Its possible role in skeletal muscle is not known.

Recently, it has been suggested that SIRT1, a NAD+-dependent histone/protein deacetylase that has been linked to increased longevity caused by calorie restriction [48], may be able to deacetylate LKB1 leading to its activation and that of AMPK in HEK (human embryonic kidney)-293T cells and rat liver in vivo [49]. The relevance of such a mechanism to AMPK activation in skeletal muscle under various conditions including exercise has not been systematically examined. However, recent studies have indicated that SIRT1 expression is increased after both a single prolonged bout of exercise [50] as well as after physical training [51]. Likewise, increases in SIRT1 mRNA have been observed in human muscle after 6 months of caloric restriction either with or without concomitant regular exercise [52]. Thus it is conceivable that under certain conditions exercise may also affect LKB1 activity in skeletal muscle and perhaps other tissues via SIRT1-induced deacetylation.

The mechanisms described above for activation of AMPK in muscle during exercise are related directly to the increase in sarcoplasmic Ca2+ concentration and the ensuing metabolic perturbations (e.g. increased AMP/ATP ratio) caused by the contractile activity (Figure 1). However, there may be additional mechanisms that increase AMPK activity in contracting muscle. For instance, increases in AMPK activity in muscle and adipose tissue caused by swimming is decreased in IL-6 (interleukin 6)-KO mice [53,54] suggesting that IL-6, which is synthesized and released by the muscle cell during sustained exercise, may be involved in AMPK activation in various tissues. Also, in keeping with this notion, it has been shown that AMPK activation in the vastus lateralis muscle of humans correlates closely with IL-6 release from the leg during ergometer cycling [55].

Exercise intensity and heterotrimeric complexes

AMPK is activated in an intensity-dependent manner, such that its activation is observed acutely at exercise intensities above ∼60% of maximal aerobic capacity [15,16,19,5658]. On the other hand, AMPK activation can also be observed if exercise at a lower intensity is very prolonged [59].

Recent findings in humans indicate that the various trimeric AMPK complexes are activated very differently during exercise. Thus, in human muscle, only three heterotrimeric AMPK complexes are expressed, α1β2γ1, α2β2γ1 and α2β2γ3, and, during intense exercise of up to 20 min duration, only the α2β2γ3 complex is activated. The other complexes, which comprise as much as 80% of the total AMPK pool are unchanged or even decreased in activity [60]. Only after moderate intensity exercise of 60 min or more does the activity of the α2β2γ1 complex increase [61]. Interestingly, increases in the activity of α2β2γ3 correlated well with increased ACCβ phosphorylation, suggesting that this trimeric complex plays a major role in the regulation of fatty acid oxidation [61]. In contrast, increases in the activity of the α2β2γ1 complex were shown to correlate with phosphorylation of the Rab-GAP (GTPase-activating protein) AS160 (Akt substrate of 160 kDa), an AMPK target that has been linked to the regulation of glucose transport [62,63]. Collectively, these findings suggest both that the regulation of the various trimeric complexes in muscle during exercise is very different and that the complexes have different downstream substrates and probably distinct biological actions.

AMPK regulation and glycogen content

In addition to exercise intensity, the magnitude of AMPK activation during exercise also depends on the content of glycogen in muscle (Figure 1). When muscle glycogen is low, AMPK activity is elevated at rest and it increases significantly more during exercise than when glycogen is high [58,6466]. This dependency on glycogen content is also apparent when AMPK is activated by AICAR (5-amino-4-imidazolecarboxamide riboside) [67,68]. Interestingly, AMPK has a glycogen-binding domain on its β-subunit [69,70]. Although previous studies have shown that glycogen does not affect AMPK activity in vitro [70], it has recently been demonstrated that glycogen inhibits purified AMPK [70a]. This effect of glycogen is dependent on the glycogen-binding domain and varies according to the degree of branching of the glycogen [70a]. These findings may explain the effects of glycogen an AMPK activity in vitro as described above.

Differences between men and women

Interestingly, AMPK activation during physical activity is less in women than in men when exercising at the same relative exercise intensity [71]. This is likely to be because women are less metabolically stressed as indicated by nucleotide status [71], possibly due to the fact that they have both a higher percentage of oxidative type I muscle fibres [72] and a greater capillary density [71] than men. Although not studied, it would seem likely that, at maximal exercise intensity, women and men would be equally stressed and presumably would have the same AMPK activation.


Glucose uptake

Activation of AMPK by AICAR in resting muscle results in increased glucose uptake [73], an effect that is lost when α2- or γ3-AMPK expression is deficient [7476]. Thus it is logical to assume that AMPK activation during exercise is responsible for the observed increase in muscle glucose uptake (Figure 3). Supportive evidence for this conclusion has been obtained in mice with various deficiencies in AMPK activity, including α1-, α2- and γ3-AMPK whole-body KO mice, muscle-specific LKB1-KO mice and transgenic mice overexpressing dominant-negative AMPK constructs in muscle as discussed below. The picture is not clear, because a partial deficiency of AMPK, such as occurs in germline α2-AMPK-KO [75] and γ3-AMPK-KO mice [74] is associated with a normal rate of glucose uptake during contractions [75]. In contrast, in mice overexpressing a dominant-negative α2-AMPK construct in muscle [44,7678] and in the muscle-specific LKB1-KO mice in which α2-AMPK activation is completely blunted [39,79], glucose uptake in muscle during electrical stimulation is impaired. In α1-AMPK-KO mice, glucose uptake during twitch contractions was shown recently to be decreased compared with that in WT mice [80], in agreement with previous studies in which a modest decrease in glucose uptake during tetanic contractions was observed in the soleus muscle of α1-AMPK-KO mice [75]. Taken together, the available data indicate that AMPK partially mediates the increase in glucose uptake during electrical stimulation of muscle. To date, all of these experiments have been performed with muscles in which contraction was induced by electrical stimulation in vitro or in situ via the sciatic nerve. It does not automatically follow that the same results would be obtained during voluntary exercise in vivo during which the muscle recruitment pattern is very different and the systemic response to exercise has to be taken into account.

Figure 3 Proposed roles of AMPK in regulation of metabolism and gene expression in skeletal muscle

Although there is clear evidence for these roles of AMPK in resting muscle, proof of this during exercise has so far been difficult to establish unequivocally using genetic mouse models with partial ablation of AMPK activity. See the text for details.

AMPK belongs to a family of ARKs (AMPK-related kinases), all of which are activated by LKB1. Although several of these ARKs {QSK, QIK (Qin-induced kinase), MARK [MAP (microtubule-associated protein)/microtubule affinity-regulating kinase] 2/3 and MARK4} do not appear to be activated during electrically induced muscle contractions [38], it was reported recently in a preliminary communication that expression of a phosphorylation impaired mutant AMPK-related kinase, SNARK (sucrose-non-fermenting protein kinase/AMP-activated protein kinase-related protein kinase) (NUAK2), blunts electrically stimulated contraction-induced glucose uptake [81].

In recent years, a direct target of Akt, AS160, has been implicated in insulin-mediated GLUT4 (glucose transporter 4) translocation and glucose uptake in both adipocytes and skeletal muscle [82,83]. It has been suggested that AS160 is also involved in the regulation of glucose transport during contraction/exercise, since it has been found to be an AMPK target in contracting muscle [63,83] (Figure 3). In addition, mutations in AS160 that prevent it from being phosphorylated decrease muscle glucose uptake during contractions [83]. Although these observations suggest an important role for AS160 in contraction-induced glucose uptake, increased muscle AS160 phosphorylation has not been observed in human muscle until after 60 min of exercise, suggesting that it may not be an initiating event [61,84]. Furthermore, recent data in incubated rat muscle showed that AS160 phosphorylation is transient despite maintained glucose uptake during 60 min of electrical stimulation [85]. To date, eight phosphorylation sites have been identified in AS160 [86]. Since the antibody (PAS) that has been used to detect phosphorylation of AS160 may bind to several, but not all, of these sites, the possibility exists that AS160 is phosphorylated on sites that are important for exercise-induced glucose transport, but are not detected by the PAS antibody. Site-specific phosphospecific antibodies will be needed to address this issue. Recently, another Akt target Tbc1d1 (Tre-2/Bub2/Cdc16 domain family, member 1) has been shown to be expressed heavily in muscle and seems to be involved in insulin-stimulated glucose uptake [87,88]. It is also phosphorylated during muscle contraction [85,88], but whether it is involved in regulating contraction-stimulated glucose uptake remains to be seen.

Fatty acid oxidation

In parallel to its effects on glucose uptake, AICAR increases fatty acid oxidation in resting skeletal muscle [73] and partly for this reason AMPK activation has been thought to participate in the regulation of fatty acid oxidation during exercise. Whole-body and muscle fatty acid oxidation increase during exercise (Figure 3) and, in particular, sustained exercise of moderate intensity [89]. However, in contrast with glucose oxidation, which increases with increasing exercise intensity [90], whole-body fatty acid oxidation seems to reach a plateau at approx. 60% of maximal aerobic capacity and at higher intensities, it then decreases [91]. On the other hand, recent evidence suggests that, when fatty acid uptake and oxidation are measured directly across a relatively small muscle group such as the vastus lateralis, fatty acid oxidation is not decreased at high exercise intensities [92], suggesting that whole-body data do not accurately reflect local muscle metabolism.

In rodents, exercise and electrically induced muscle contractions decrease the concentration of malonyl-CoA in muscle [19,93,94] presumably due to the phosphorylation and inhibition by AMPK of ACCβ, the ACC isoform which catalyses the synthesis of the pool of malonyl-CoA that inhibits CPT1 (carnitine palmitoyltransferase 1) [95,96]. Also, the phosphorylation and activation of malonyl-CoA decarboxylase by AMPK and perhaps other factors operative during exercise could enhance this effect [94]. Initial studies failed to find decreases in muscle malonyl-CoA during exercise in humans [97,98]; however, more recent studies have described modest decreases in its concentration in human muscle after both acute exercise [99,100] and physical training [101]. Although AMPK activation and the ensuing decrease in malonyl-CoA concentration in muscle may be an important mediator of the increase in muscle fatty acid oxidation during the transition from rest to exercise, it does not seem to play a key regulatory role during exercise. Thus, during exercise of increasing intensity, muscle AMPK activity increases [15], but, as mentioned above, whole-body fatty acid oxidation decreases [91] or remains stable when measured across an exercising muscle [92]. Furthermore, when muscle glycogen stores are decreased before exercise, muscle lipid oxidation is greater than when exercise is performed by muscle with full glycogen stores, but malonyl-CoA concentrations are similar [100]. Recent studies in mice overexpressing a kinase-dead α2-AMPK construct in heart and skeletal muscle showed that, during both in vitro electrical stimulation of muscle and in vivo exercise, impaired AMPK signalling was not accompanied by decreased fatty acid oxidation [102]. Data obtained in perfused rat skeletal muscle has suggested an important role for Ca2+ signalling and ERK (extracellular-signal-regulated kinase) activation in regulating fatty acid uptake and oxidation during electrically induced muscle contractions [103,104]. Thus fine-tuning of lipid oxidation may not be provided by AMPK. Other studies suggest that such fine-tuning takes place at the level of carnitine availability, which, like AMPK, is regulated by exercise intensity and carbohydrate availability [100,105]. A low level of free carnitine in muscle limits the possibility for CPT1-catalysed conversion of LCFAs (long-chain fatty acids) into fatty acylcarnitines in which form they can enter the mitochondria for oxidation.

AMPK activation during exercise may also decrease triacylglycerol synthesis in adipose tissue and liver by inhibiting the enzyme GPAT (sn-glycerol-3-phosphate acyltransferase) [19,106]. In addition, activation of AMPK in isolated cardiomyocytes results in translocation of the putative fatty acid transporter FAT/CD36 to the cell membrane which could increase fatty acid uptake as well as its oxidation [107]. In keeping with this possibility, AMPK stimulation in resting muscle has been shown to increase fatty acid oxidation in a CD36-dependent manner [108]. Thus it appears that AMPK activation during exercise affects multiple enzymes and other molecules to increase the uptake as well as the oxidation of fatty acids.

Protein synthesis

Protein synthesis is markedly diminished in skeletal muscle during contraction [109111] (Figure 3). It has been proposed that AMPK activation inhibits protein synthesis in liver and ischaemic heart muscle by activating eEF2 (eukaryotic elongation factor 2) kinase, leading to increased eEF2 phosphorylation and inhibition of peptide elongation [112,113]. Inhibition of peptide elongation in muscle during exercise has also been suggested by the finding of a rapid increase in eEF2 phosphorylation at the onset of bicycle ergometer exercise [114]. This appears to precede an increase in AMPK activity and is probably due to Ca2+-dependent CaMKIII (eEF2 kinase) activation [114]. Interestingly, immediately following strength training, increased eEF2 phosphorylation has not been observed [111,115]. The reason for the different responses after endurance exercise and strength training is unclear. In addition to inhibition of peptide elongation, translation initiation may also be inhibited during exercise, since decreased phosphorylation of 4EBP1 (eukaryotic initiation factor 4E-binding protein 1) in muscle has been described both during resistance exercise training [111,115] and endurance exercise [116], perhaps due to increased α2-AMPK activity and TSC2 (tuberous sclerosis complex 2) phosphorylation (Figure 4).

Figure 4 Model describing the regulation of protein synthesis by AMPK, Ca2+ and Akt signalling pathways

Phosphorylation of TSC2 by Akt induces activation of the Rheb GTPase and the mTORC1 (mammalian target of rapamycin complex 1) pathway, promoting protein synthesis. In contrast, phosphorylation of TSC2 by AMPK results in Rheb inactivation and mTORC1 inhibition. Activated mTORC1 phosphorylates and activates p70S6K (p70 S6 kinase) and phosphorylates and inactivates eEF2 kinase (an inhibitor of eEF2), resulting respectively in the stimulation of peptide translation and elongation. In addition, activated mTORC1 phosphorylates 4EBP1, which binds to and inhibits the initiation factor eIF4E (eukaryotic initiation factor 4E). This in turn inhibits 4EBP1 binding to eIF4E and enhances its ability to initiate translation. Thus mTORC1 co-ordinates the regulation of protein synthesis at the levels of initiation, translation and elongation. The same colours indicate the same level in signal transduction.

In contrast with the inhibition of protein synthesis during exercise, after a bout of exercise, protein synthesis is typically increased [111,117] (as is glycogen synthesis), despite persistent increases in AMPK activity [111], casting doubt about the primacy of AMPK in regulating protein synthesis in human skeletal muscle in this situation. In rodents and humans, the sensitivity to insulin of several metabolic pathways, including protein [118] and glycogen synthesis [21], amino acid transport [119] and glucose uptake [21,22] are increased after exercise, and perhaps this compensates for the increase in AMPK activity. In agreement with this interpretation, it has been shown recently that elements of the molecular pathway controlling peptide elongation are increased during insulin stimulation 4 h after one-legged endurance exercise, compared with the non-exercised control leg [120].

Decreased running performance in AMPK-deficient mouse muscle

Although it has been difficult to pinpoint defects in substrate metabolism, there is no doubt that mice with defects in AMPK activation, such as muscle-specific kinase-dead mice [121,122], α2-AMPK germline KO mice [123] and LKB1 muscle-specific KO mice [40], have poor running capacity in vivo and disturbed nucleotide levels in muscle during exercise/contraction [79,122125]. Thus a partial absence of AMPK in muscle somehow results in poor exercise performance. The reason for this is not immediately obvious, but could be related to decreased mitochondrial capacity in the muscles of these mice, rather than to an acute effect on metabolic regulation [5,40,122,125]. However, despite the decreased running performance, mice that overexpress a dominant-negative α2-AMPK construct in muscle did not display altered fuel oxidation compared with wild-type mice as evaluated by respiratory quotient and oxygen uptake [102]. The possibility that decreased running performance in these mice is the result of decreased cardiac performance during exercise also cannot be excluded, since the genetic abnormalities in these mice also target the heart. On the other hand, this possibility seems less likely, since heart-specific AMPK dominant-negative mice appear to have a normal exercise capacity [126].


Liver and adipose tissue

It has long been appreciated that an acute bout of exercise is associated with changes in the metabolism of liver and adipose tissue that result in an increased provision of fuel for the contracting muscle. Thus the liver increases the release into the circulation of glucose (initially derived from glycogenolysis and later from gluconeogenesis) and the adipocyte increases the hydrolysis of its triacylglycerol stores and the release of LCFAs into the circulation. A large body of evidence from both humans and experimental animals has linked these changes temporally to activation of the sympathetic nervous system (noradrenaline), which occurs very early during moderate and intense exercise and later to both this and increases in plasma levels of glucagon and adrenaline (for a review, see [127]). In addition, IL-6 released from muscle during exercise may stimulate the hydrolysis of fuel reservoirs in muscle, liver and adipose tissue, and causes AMPK activation in these tissues (see below).

AMPK activation decreases the expression of PEPCK (phosphoenolpyruvate carboxykinase) and glucose-6-phosphatase in the liver, two rate-limiting enzymes of the gluconeogenic pathway (for a review, see [128]). In addition, AMPK appears to inhibit hormone-sensitive lipase (reviewed in [129]), a key enzyme that controls lipolysis in the fat cell and other tissues. Thus it was surprising when studies in rodents revealed that exercise activates rather than inhibits AMPK in liver [19,130] and intra-abdominal adipose tissue [19,131], since AMPK activation should theoretically have inhibited gluconeogenesis and lipolysis. In both tissues, exercise also caused a decrease in the energy state as reflected by an increase in the AMP/ATP ratio [130,132]. The most plausible explanation for the findings in liver is that the increase in the AMP/ATP ratio caused by exercise reflects the high energy cost of gluconeogenesis (six ATPs per mol of glucose generated from lactate) and that AMPK acts acutely (i.e. before its effects on gluconeogenic gene expression becomes dominant) to maintain gluconeogenesis by enhancing ATP generation from fatty acid oxidation. A similar line of reasoning could explain the findings in adipose tissue. Thus recent studies with cultured adipocytes have demonstrated that the stimulation of lipolysis by agents that increase cAMP generation produce the same changes in AMPK activity and energy state in these cells [133], as does exercise in vivo [132]. It was shown that inhibition of lipolysis with an siRNA (small interfering RNA) for ATGL (adipose tissue triacylglycerol lipase) or a chemical inhibitor (orlistat) prevented both of these changes from occurring, as did incubation with triacsin C, an inhibitor of acyl-CoA synthetase [133]. On the basis of these findings, it was postulated that the decrease in energy state in these cells was due to the high ATP requirement for fatty acid re-esterification (seven to nine ATP molecules of triacylglycerol resynthesized), since 30–40% of the non-esterified fatty acids generated by lipolysis in a fat cell is re-esterified even when the lipolytic rate is increased. Interestingly, chemical inhibition of AMPK with compound C [133] or treatment of the adipocytes with siRNA for AMPK (M.S. Gauthier and N. Ruderman, unpublished work) caused a large increase in oxidant stress which increased markedly when lipolysis was stimulated. Presumably in this setting AMPK acts to restore cellular energy state and prevent lipotoxicity. Whether it does this by increasing fatty acid oxidation, inhibiting re-esterification or modestly restraining lipolysis remains to be determined [133]. Also requiring study is whether a failure of AMPK to restore the energy state of the adipocyte predisposes it to apoptosis and inflammation [134].

A number of aspects of the acute-exercise-induced changes in AMPK and energy state observed in rodent liver and epididymal adipose tissue require further study. One of them is whether similar phenomena occur in humans As noted earlier, in one study, exercise of moderate to high intensity did not activate AMPK in human subcutaneous adipose tissue [135], whereas a small increase in phosphorylation of AMPK was reported in another [136]. Studies in rats suggest that long-term exercise stimulates AMPK activity (and subunit expression) in liver and in intra-abdominal, but not subcutaneous, adipose tissue [137]. Whether a similar difference in adipose tissue occurs in humans is not known. Also requiring investigation is whether factors other than the sympathetic nervous system contribute to these exercise-induced changes in AMPK activity. In this context, it has been demonstrated that the cytokine IL-6 is synthesized and released by skeletal muscle of both humans and rodents during prolonged exercise [138] and that in humans an IL-6 infusion to simulate this effect increased adipose tissue lipolysis [139]. Of specific relevance to this discussion, IL-6 administration both in vivo and in vitro has been shown to activate AMPK activity in both skeletal muscle and adipose tissue of rodents [54]. In addition, in IL-6-KO mice, the baseline activity of AMPK in muscle and epididymal adipose tissue is substantially diminished, and the increase in AMPK activity caused by exercise is attenuated, although not completely prevented [131].


It has been demonstrated that leptin diminishes AMPK activity in the hypothalamus and that this is responsible for its ability to decrease food intake and increase sympathetic nervous system activity in the periphery [8,140]. Conversely, endocannabinoids [141] and ghrelin [142] increase hypothalamic AMPK activity and food intake. In an early study [143], an effect of exercise on AMPK activity in the hypothalamus was not observed. On the other hand, Flores et al. [144] later reported that exercise increases the ability of leptin and insulin, acting at the level of the hypothalamus, to diminish food intake, and Park et al. [145] have shown that exercise prevents dexamethasone-induced impairment of insulin and leptin signalling in the hypothalamus and, in so doing, reverses the phosphorylation of AMPK induced by dexamethasone. How exercise achieves these effects is not known.


Skeletal muscle

In seminal studies, Winder's group demonstrated that repeated activation of AMPK by daily injections of AICAR in rats increased GLUT4 and hexokinase protein content, and the expression of several mitochondrial enzymes in skeletal muscle [4,146]. Subsequently, it was shown that the feeding of mice with GPA (β-guanidinopropionic acid), a creatine analogue that leads to increases in the intramuscular AMP/ATP ratio and AMPK activity, increased mitochondrial biogenesis in control mice, but not in mice overexpressing a dominant-negative AMPK in muscle [147]. These adaptations resemble those observed in skeletal muscle during endurance training and suggest that AMPK may play a key role in their causation. Other evidence speaks against this hypothesis, however. Thus exercise training-induced increases in GLUT4 protein and mitochondrial enzymes were normal in both mice overexpressing a dominant-negative AMPK construct in muscle [148] and mice with a germline deletion of α2-AMPK [5]. In addition, in the latter, exercise-induced increases in the mRNA for a number of mitochondrial enzymes were similar to those of WT mice [124]. On the other hand, both in these mice [5,125] and mice overexpressing the dominant-negative AMPK construct [148], mitochondrial protein expression was decreased by 15–20% in the untrained basal state, suggesting that AMPK activity is important for basal mitochondrial enzyme expression. In contrast with these findings, muscle fibre type expression does not seem to be significantly altered under baseline conditions in mice overexpressing a dominant-negative AMPK construct in muscle [148,149]. On the other hand, the switch to a more oxidative muscle fibre type elicited by endurance training was inhibited in these mice [148] and in mice overexpressing a constitutively active mutant γ1-AMPK in muscle, it has been shown that the fibre type composition of the gastrocnemius became more oxidative [148]. In summary, the available information suggests that in the basal state when its activity is low, AMPK appears to mediate changes in mitochondrial protein expression, but not fibre type. In contrast, when AMPK activity is high, such as after exercise training or overexpression of a constitutively active AMPK, it appears to regulate muscle fibre type. This moderate effect on muscle fibre type expression is not too surprising considering that regulation of muscle fibre type expression is very complex involving several other signalling molecules (e.g. calcineurin, CaMK and p38), as well as transcription factors and co-activators {e.g. PGC-1α [(PPAR (peroxisome-proliferator-activated receptor) γ co-activator 1α], NFAT (nuclear factor of activated T-cells) and PPARδ} [150].

AMPK abundance itself may be influenced in muscle by endurance training. In humans, trained subjects have a higher expression of α1-AMPK than untrained individuals [151]. Furthermore, intense endurance training of young healthy males for 3 weeks results in increases in α1- and α2-AMPK protein expression and ACCβ phosphorylation; the latter strongly suggesting that the basal activity of AMPK was increased [152]. Interestingly, in contrast with these changes, γ3-subunit expression was decreased by training [152]. Increased AMPK activity, which can persist in trained individuals for some time between exercise bouts, may be important in eliciting increased insulin sensitivity, since AMPK activation by AICAR has been shown to increase insulin action in rat muscle [153,154]. In middle-aged subjects, less intense training did not reveal changes in AMPK protein expression and activity. When studied 24–36 h after the last bout of exercise, however, PGC-1α and malonyl-CoA decarboxylase mRNA expression, both of which can be AMPK-mediated, were increased for at least 24–30 h [101].

Other tissues

Regular exercise leads to decreased inflammation, increased fibrinolytic activity, lowering of plasma triacylglycerols and increased HDL (high-density lipoprotein) cholesterol [10]. To what extent these are chronic effects of repeated AMPK activation in tissues other than muscle is essentially an unanswered question. As already noted, long-term exercise training (6 or 12 weeks) induces increases in AMPK and ACC phosphorylation and α1- and α2-AMPK mRNA and protein in both rat visceral adipose tissue and liver [132,137]. Acutely, exercise-induced AMPK activation in these tissues is associated with decreases in the activities of a number of enzymes of lipid synthesis, including glycerophosphate acyltransferase and acetyl-CoA carboxylase, and with an increase in malonyl-CoA decarboxylase activity [19]. The cumulative effect of these changes would be an increase in fatty acid oxidation and a decrease in glycerolipid synthesis. Presumably, this occurs in liver and adipose tissue during physical training; however, to our knowledge this has not been studied directly.


As already noted, inactivity is associated with an increased prevalence of various disorders, including Type 2 diabetes, coronary heart disease, Alzheimer's disease and cancers of the colon and liver [155,156]. In addition, it is well established that bed rest for 3–7 days leads to glucose intolerance and insulin resistance [157159]. Whether inactivity also leads to decreased activation of AMPK-mediated genes such as PGC-1α [50,160] is not known. A less dramatic decrease in physical activity than with bed rest occurs when healthy free-living subjects decrease their daily number of steps from 6000–10000 to 1500 [161]. It was shown in this study that within 2–3 weeks of reduced stepping, oral glucose tolerance and insulin response to an OGTT (oral glucose tolerance test) were both decreased and plasma triacylglycerols were increased [161]. The relationship of such abnormalities to AMPK dysregulation is unclear. The predicted defect in AMPK in response to inactivity would be the absence of intermittent periods of AMPK activation caused by physical activity and possibly decreased AMPK activity at rest.


Exercise, AMPK and the metabolic syndrome

The association between exercise, AMPK activation and disease prevention will be discussed primarily in the context of the metabolic syndrome. The metabolic syndrome is presently defined clinically as a disorder characterized by at least three of the following: central obesity, hyperglycaemia, hypertension, hypertriglyceridaemia and a decrease in circulating levels of HDL cholesterol [162]. In addition, patients with this entity are typically insulin-resistant and many present with a pro-inflammatory procoagulant state [increased IL-6, TNFα (tumour necrosis factor α) and PAI (plasminogen-activator inhibitor)], all of which may antedate the clinical diagnosis by many years and contribute to its pathogenesis (Figure 5). Patients with the metabolic syndrome are predisposed to such disorders as Type 2 diabetes, premature atherosclerotic heart disease, non-alcoholic fatty liver disease, various cancers [10] and neurodegenerative disorders, including Alzheimer's and Parkinson's disease, all of which are less prevalent in individuals who are physically active [156,163165]. As reviewed elsewhere [166168], regular exercise has also been shown to increase insulin-sensitivity and decrease inflammatory markers in people with the metabolic syndrome. In addition, in conjunction with diet therapy, exercise markedly diminishes progression from impaired glucose tolerance to overt diabetes in humans studied over 4–5 years [169171] and it has shown efficacy in the prevention or therapy of many other metabolic-syndrome-associated disorders (Figure 6). The linkage of these effects of exercise to AMPK has not been systematically examined. However, it is noteworthy that treatment with metformin and thiazolidinediones, antidiabetic agents that activate AMPK in human muscle [172,173] and diminish systemic insulin resistance (as does exercise) have also been demonstrated to decrease both progression from impaired glucose tolerance to overt diabetes [169,170,174] and risk factors for atherosclerotic heart disease [175]. In addition, metformin has been demonstrated to diminish the incidence of macrovascular disease by 39% when used as the sole initial therapy in individuals with new-onset Type 2 diabetes in the UKPDS (UK Prospective Diabetes Study) [176], although, in the same study, a significantly higher incidence of macrovascular disease was observed in individuals treated with sulfonylureas and metformin [176]. More recently, a 35% decrease in cancer prevalence has been reported in patients with diabetes treated with metformin for many years [177,178], suggesting that AMPK may act as a tumour suppressor [11]. Regular exercise is known to be protective against some forms of cancer, including cancer of the breast, colon and probably the prostate [179], and the possibility exists that activation of AMPK by exercise may be involved in this association.

Figure 5 The metabolic syndrome

The metabolic syndrome can be defined as a disorder in which combinations of overnutrition, inactivity and as yet poorly defined genetic factors results in a state of metabolic dysregulation characterized by lipid abnormalities, insulin resistance and inflammation. This in turn can predispose to one or more of the shown disorders. Apart from inactivity being a causal factor, exercise has demonstrated efficacy in treating and preventing both the state of metabolic dysregulation and many of the diseases to which it predisposes. See the text for details. ASCVD, atherosclerotic cardiovascular disease; PCOS, polycystic ovary syndrome.

Figure 6 Some metabolic-syndrome-associated disorders that may be improved or prevented by exercise

Exercise, acting at least in part via AMPK, could exert these benefits by improving abnormalities in glucose and lipid metabolism, insulin secretion and action, inflammation, mitochondrial function, angiogenesis and other pathogenic factors. ASCVD, atherosclerotic cardiovascular disease; T2DM, Type 2 diabetes mellitus.

Obesity, insulin resistance and Type 2 diabetes

It must be emphasized that measurements of AMPK activity in humans with obesity and insulin resistance, with or without diabetes, have not painted a clear picture. Early studies from several laboratories failed to find abnormalities in AMPK activity in skeletal muscle of these patient groups [172,180]. Furthermore, no defect in the ability of AMP and AICAR to activate AMPK was observed in patients with obesity [181] and Type 2 diabetes [182]. On the other hand, it has been reported recently that the ability of exercise to activate AMPK is impaired in muscle of obese individuals with or without diabetes [183,184]. In one of these studies [184], increases in PGC-1α mRNA and abundance induced by an acute bout of exercise in lean control subjects were markedly diminished in obese individuals; however, in the other [183], baseline PGC-1α mRNA levels were diminished, but the percentage increase induced by exercise was the same as in the control population. Likewise, decreased AMPK activity accompanied by increases in malonyl-CoA and ACC activity (decreased phosphorylated ACC) have been reported in obese insulin-resistant humans with and without diabetes [173]. Interestingly, treatment with rosiglitazone (a thiazolidinedione) activated AMPK and diminished both the concentration of malonyl-CoA and insulin resistance, as it had previously been reported to do in obese rodents [185,186]. Further studies are needed to confirm and extend these findings.

AMPK, exercise and the metabolic syndrome phenotype in rodents

Investigations in rodents are consistent with the notion that exercise may prevent disease by activating AMPK, although a causal role has not been established. Early studies by Berger and co-workers demonstrated that exercise diminishes insulin resistance and obesity in the Zucker fatty rat (fa/fa) [187], a rodent later found to have a genetically defective leptin receptor [188] and a decrease in tissue AMPK activity [189]. As shown in Table 1, decreased tissue AMPK activity has been found in many rodents with a metabolic syndrome phenotype. One of these is the ZDF (Zucker diabetic fatty) rat, an obese rodent with the same genetic defect in the leptin receptor as the fa/fa rat and with a second as yet undefined genetic defect that causes it to develop diabetes between 10 and 13 weeks of age. Treatment of the ZDF rat with the AMPK activator AICAR [189,190] or with regular exercise [190], beginning at 5 weeks of age, has been shown to diminish insulin resistance and ectopic lipid deposition in liver, muscle and pancreatic islets and prevent the development of diabetes in the fa/fa rat. Similar changes have been observed in mice subjected to caloric restriction, or treated with thiazolidinediones or metformin [188], suggesting that diminished AMPK activity is a pathogenetic factor.

View this table:
Table 1 Some rodents in which decreased AMPK activity is associated with the metabolic syndrome

Another animal of note is the IL-6-KO mouse. The IL-6-KO mouse appears normal at 3 months of age, but has low AMPK activity in white muscle and adipose tissue [131], a low rate of fatty acid oxidation and an impaired ability to sustain exercise [191]. By 7–9 months of age, however, it is obese, glucose-intolerant and dyslipidaemic [191], and fatty acid oxidation has retuned to the same level as that in control mice, although its ability to exercise is still impaired [192]. Whether treatment with IL-6, which activates AMPK in muscle and adipose tissue both in vivo and in vitro [54], can prevent these changes from occurring is not known. Studies by Wallenius et al. [191] suggested that intraperitoneally injected IL-6 has little effect on the phenotype of these mice, but that IL-6 administered into the third ventricle increased energy expenditure and diminished their obesity. Of final note, IL-6 is synthesized and released from skeletal muscle during exercise and its concentration in plasma can increase transiently (hours) as much as 100-fold [138] when running a marathon race, although much more modest increases are observed during more moderate exercise bouts [55]. In the IL-6-KO mouse, this increase in IL-6 synthesis during exercise does not occur, and the activation of AMPK in both muscle and adipose tissue is markedly, although not totally, impaired [131].

In the mice with a metabolic syndrome phenotype listed in Table 1, the decreases in AMPK activity were all secondary to another factor, such as a defective leptin receptor, or an oversupply of glucose or other nutrients. Studies of mice with a germline deletion of α1- or α2-AMPK to date have not yielded similar phenotypes, although the α2-AMPK-KO mouse is insulin-resistant in vivo, possibly the result of an increase in circulating catecholamines due to a decrease in hypothalamic AMPK [193]. Whether a second stress, such as age or prolonged overnutrition, is needed for a metabolic syndrome phenotype to occur is not known. Overfeeding on a high-fat diet has been found to cause a greater increase in obesity in mice with a germline α2-AMPK deficiency; however, it failed to produce insulin resistance as it did in control mice [194] for reasons unknown. In contrast, in mice with a muscle-specific α2-AMPK ablation, a similar high-fat diet caused substantially more glucose intolerance and insulin resistance in muscle (Akt phosphorylation) than it did in control mice [195], indicating that ablation of α2-AMPK activity in muscle in fact exacerbates sensitivity to the deleterious effects of a high-fat diet. Why muscle-specific deletion of α2-AMPK has this effect when germline deletion of α2-AMPK does not is at present unclear.

Some less studied disorders that are improved by physical activity

As reviewed elsewhere [10,14], the metabolic syndrome has been linked to a wide array of diseases in addition to those already described, including NAFLD (non-alcoholic fatty liver disease)/NASH (non-alcoholic steatohepatitis), polycystic ovary syndrome and cancers of the colon, breast, prostate and uterus. Furthermore, exercise has shown benefit in the prevention and therapy of many of these disorders (Figure 6). In the following sections, we discuss two of the disorders that have been more recently linked both to exercise and AMPK: capillary rarefaction and Alzheimer's disease.

Capillary rarefaction

Decreased capillary density in skeletal muscle (rarefaction) has been described in humans with obesity, diabetes and inactivity, and it can be at least partially reversed by caloric restriction and exercise [196,197]. Similar findings have been observed in rodents. The most studied rodent has been the obese Zucker rat (fa/fa) in which a 75% decrease in muscle capillary density at age 6–7 weeks was substantially (50–60%) corrected after 10 weeks of regular exercise (treadmill running for 1 h/day, 6 days a week at a rate of ∼22 m/min) [198]. Interestingly, AMPK activity is diminished in several tissues of the fa/fa rat [189]. AMPK has been implicated in the regulation of angiogenesis in response to ischaemia by Walsh and co-workers [199], and others have demonstrated an association between impaired angiogenesis and capillary rarefaction. Impaired angiogenesis in skeletal muscle of mice with experimental diabetes is associated with a decrease in the expression of VEGF (vascular endothelial growth factor) [200], which can activate AMPK [201]. Exercise increases NO (nitric oxide) generation and eNOS (endothelial nitric oxide synthase) mRNA in skeletal and cardiac muscle [202], possibly via AMPK activation [203,204], and its ability to increase angiogenesis and muscle capillary density is lost following treatment with eNOS inhibitors [198]. Likewise, the histone/protein deacetylase SIRT1 has been shown to stimulate NO production by endothelial cells [205], and a lack of SIRT1 impairs angiogenesis [206]. In addition, exercise has been shown to increase SIRT1 activity and antioxidant enzymes in the heart of aging rats [51]. As discussed above, SIRT1 may be an upstream regulator of AMPK. It will therefore be of interest to determine whether the link between SIRT1 activity and capillarity is AMPK-mediated.

Alzheimer's disease and dementia

Alzheimer's disease and other neurodegenerative disorders that lead to dementia are more prevalent in people with the metabolic syndrome and Type 2 diabetes than in the general population [163]. Although the evidence is not universally consistent, both observational and prospective studies strongly suggest that the risk for these disorders is diminished in humans who are more physically active [207]. In addition, improvement in cognitive function by physical activity has recently been observed in a randomized prospective trial in older adults at risk of Alzheimer's disease [208].

Alzheimer's disease has been linked to such factors as insulin resistance in the brain [207] and loss of neurons in the parietal and temporal lobes including the hippocampus [207]. In a recent study [209], 3 months of aerobic training in humans was associated with MRI (magnetic resonance imaging) measures of increased cerebral blood volume (and presumably blood flow) in the dentate gyrus of the hippocampus. Likewise, in mice, exercise increased neurogenesis in the dentate gyrus [209]. Other studies in rodents have shown that regular exercise: (i) has cognitive effects similar to those it has in humans [207]; (ii) increases mitochondrial biogenesis and decreases various manifestations of oxidative stress in the brain [210]; (iii) diminishes cerebral inflammation in the Tg2576 mouse, a rodent model of Alzheimer's disease [211]; and (iv) increases the synthesis and/or effects of BDNF (brain-derived neurotrophic factor), IGF-1 (insulin-like growth factor-1) and VEGF, which collectively result in neuroprotection, angiogenesis, cell proliferation and changes in cortical morphology [207]. AMPK in brain was not measured in these studies; however, its activation has been shown to cause similar effects in other tissues. Finally, caloric restriction (60% of control), which has been shown to activate AMPK in the hippocampus of the rat, has also been found to increase neurogenesis and improve cognition [212].

SIRT1, a histone/protein deacetylase that has been linked to the increase in longevity associated with caloric restriction, has also been reported to provide neuronal protection in rodents with Alzheimer's disease [213,214]. In some of these studies, resveratrol, a SIRT1 activator, was demonstrated to diminish neurodegeneration in rodent models of both Alzheimer's [214,215] and Huntington's [213] disease. Recent reports suggest that SIRT1 may function, at least in part, by activating AMPK [216,217]; indeed, resveratrol, which was used in some of the above-mentioned rodent studies [213,218], has clearly been demonstrated to activate AMPK in various tissues and cells [217,218]. On the other hand, the role of SIRT1 in mediating the neuroprotective effect of resveratrol has recently been questioned [219]. Thus both resveratrol and the AMPK activator AICAR promoted robust neurite outgrowth in Neuro2A cells, and the effects of both agents were blocked by compound C, a compound commonly used to inhibit AMPK. However, the effects of resveratrol were still evident in cells lacking SIRT1 [219]. Whether this signifies that resveratrol does not require a sirtuin to activate AMPK (e.g. at high concentrations, it can inhibit mitochondrial ATP synthase), or another sirtuin assumed its function in these cells remains to be determined. With regard to the latter possibility, resveratrol has been shown to activate SIRT2 in neuronal cells [220]. In any event, studies of the effect of exercise on SIRT1 and AMPK activity in various regions of the brain will clearly be of interest.


Physical activity has unequivocal health benefits related to the prevention and treatment of lifestyle disorders associated with obesity and insulin resistance, including Type 2 diabetes, cardiovascular and neurodegenerative diseases, and some cancers. In addition, regular physical activity is associated with decreases in all causes of mortality. That AMPK plays a key role in mediating these benefits of exercise has been suggested. The fact that exercise causes activation of AMPK in muscle and, at least in rodents, adipose tissue and liver, coupled with the observation that pharmacological activation of AMPK leads to marked metabolic improvements in animal models of the metabolic syndrome has led to the assumption that its activation by exercise is a major reason for these health-promoting effects of physical activity. Also in support of this notion, decreased AMPK activity has been shown to accompany or precede the appearance of a metabolic syndrome phenotype in many of these rodents. The situation in humans is less clear. As already noted, both normal and decreased AMPK activity and normal and impaired activation of AMPK by exercise have been reported in obese individuals both with and without diabetes, and the reason for these differing results is unclear. Likewise, the assumption that, in sedentary people, the lack of even modest AMPK activation could have long-term consequences for mitochondrial function and other events that lead to cellular dysfunction and disease requires further study. Studies of genetic mouse models with partial deficiencies in AMPK (total KO of AMPK leads to embryonic death) have so far not consistently demonstrated that these mice become insulin-resistant or are more prone to obesity, diabetes and other disorders associated with the metabolic syndrome. However, germline-deficient mouse models may not adequately reflect the effect of the loss of AMPK protein, since compensatory mechanisms may operate from conception. In addition, decreased AMPK activity in the hypothalamus could hypothetically mask some of the effects of peripheral AMPK deficiency by increasing sympathetic nervous system activity. Organ-specific knockdown of AMPK may prove useful in addressing this issue. Indeed, as already noted, in a recent study, an increase in insulin resistance compared with control animals was observed in mice with a muscle-specific ablation of α2-AMPK when fed on a high-fat diet for an extended time period [195]. Finally, in humans, there is still a significant need to clarify the role of AMPK in regulating physiological responses in health and disease and, in particular, the response to exercise. We predict that future research will address questions such as: how significant are exercise-induced increases in AMPK activity in regulating the metabolism and function of muscle and tissues other than muscle; does dysfunction of AMPK play a role in the pathogenesis of insulin resistance and Type 2 diabetes; is AMPK a target for the prevention and treatment of Type 2 diabetes, Alzheimer's disease and certain cancers; and what is the precise physiological role of the sirtuins and their relationship with AMPK?


E. A. R. is supported by grants from the Danish Medical Research Council, the Lundbeck Foundation, Novo-Nordisk Research Foundation, the European Commission Grant COST (European Cooperation in the field of Scientific and Technical Research) BM 0602 and by an Integrated Project EXGENESIS funded by the European Union. N. B. R. is supported by the U.S. Public Health Service [grant numbers RO1 DK19514, 67509 and PO1 HL 68758] and a Mentor-based training grant from the American Diabetes Association [grant number 7-08-MN-50].


We thank Dr Laurie Goodyear for critically reviewing the manuscript and for her many helpful suggestions.

Abbreviations: ACC, acetyl-CoA carboxylase; AICAR, 5-amino-4-imidazolecarboxamide riboside; AMPK, AMP-activated protein kinase; ARK, AMPK-related kinase; AS160, Akt substrate of 160 kDa; CaMK, Ca2+/calmodulin-dependent protein kinase; CaMKK, CaMK kinase; CPT1, carnitine palmitoyltransferase 1; 4EBP1, eukaryotic initiation factor 4E-binding protein 1; eEF2, eukaryotic elongation factor 2; eNOS, endothelial nitric oxide synthase; GLUT4, glucose transporter 4; HDL, high-density lipoprotein; IL-6, interleukin 6; KO, knockout; LCFA, long-chain fatty acid; MARK, MAP (microtubule-associated protein)/microtubule affinity-regulating kinase; NAFLD, non-alcoholic fatty liver disease; NASH, non-alcoholic steatohepatitis; PPAR, peroxisome-proliferator-activated receptor; PGC-1α, PPARγ co-activator 1α; siRNA, small interfering RNA; SIRT1, silent information regulator 1; TAK1, TGF (transforming growth factor)-β-activated kinase 1; TSC, tuberous sclerosis complex; VEGF, vascular endothelial growth factor; WT, wild-type; ZDF, Zucker diabetic fatty


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View Abstract