Yeast cells Saccharomyces cerevisiae, late embryogenic abundant-like stress response protein Hsp 12 (heat-shock protein 12) were found by immunocytochemistry to be located both in the cytoplasm and in the cell wall, from where they could be extracted with dilute NaOH solutions. Yeast cells with the Hsp 12 gene disrupted were unable to grow in the presence of either 12 mM caffeine or 0.43 mM Congo Red, molecules known to affect cell-wall integrity. The volume of yeast cells were less affected by rapid changes in the osmolality of the growth medium when compared with the wild-type yeast cells, suggesting a role for Hsp 12 in the flexibility of the cell wall. This was also suggested by subjecting the yeast cells to rapid changes in barometric pressure where it was found that wild-type yeast cells were more resistant to cellular breakage.
- alkaline extraction
- heat-shock protein 12 (Hsp 12)
- late-embryonic-abundant-like protein (LEA-like protein)
- Saccharomyces cerevisiae
Abbreviations used: GFP, green fluorescent protein; Hsp, heat-shock protein; LEA, late embryogenic abundant; YEPD, yeast extract peptone dextrose.
- The Biochemical Society, London ©2004