Biochemical Journal

Research article

Structural analysis of xylanase inhibitor protein I (XIP-I), a proteinaceous xylanase inhibitor from wheat (Triticum aestivum, var. Soisson)

Françoise PAYAN, Ruth FLATMAN, Sophie PORCIERO, Gary WILLIAMSON, Nathalie JUGE, Alain ROUSSEL

Abstract

A novel class of proteinaceous inhibitors exhibiting specificity towards microbial xylanases has recently been discovered in cereals. The three-dimensional structure of xylanase inhibitor protein I (XIP-I) from wheat (Triticum aestivum, var. Soisson) was determined by X-ray crystallography at 1.8 Å (1 Å=0.1 nm) resolution. The inhibitor possesses a (β/α)8 barrel fold and has structural features typical of glycoside hydrolase family 18, namely two consensus regions, approximately corresponding to the third and fourth barrel strands, and two non-proline cis-peptide bonds, Ser36–Phe and Trp256–Asp (in XIP-I numbering). However, detailed structural analysis of XIP-I revealed several differences in the region homologous with the active site of chitinases. The catalytic glutamic acid residue of family 18 chitinases [Glu127 in hevamine, a chitinase/lysozyme from the rubber tree (Hevea brasiliensis)] is conserved in the structure of the inhibitor (Glu128), but its side chain is fully engaged in salt bridges with two neighbouring arginine residues. Gly81, located in subsite −1 of hevamine, where the reaction intermediate is formed, is replaced by Tyr80 in XIP-I. The tyrosine side chain fills the subsite area and makes a strong hydrogen bond with the side chain of Glu190 located at the opposite side of the cleft, preventing access of the substrate to the catalytic glutamic acid. The structural differences in the inhibitor cleft structure probably account for the lack of activity of XIP-I towards chitin.

  • family 18 chitinase
  • hevamine
  • wheat
  • X-ray structure
  • xylanase inhibitor

Footnotes

  • 2 Present address: Nestlé Research Centre, Vers-Chez-Les-Blanc, PO Box 44, CH-1000 Lausanne 26, Switzerland.

  • Abbreviations used: GH, glycoside hydrolase; r.m.s. root mean square; tri-NAG, N,N′,N″-triacetylchitotriose; XIP-I, xylanase inhibitor protein I.