Biochemical Journal

Research article

MIM-B, a putative metastasis suppressor protein, binds to actin and to protein tyrosine phosphatase delta

Jacquelyn A. WOODINGS, Stewart J. SHARP, Laura M. MACHESKY

Abstract

We have found that MIM-B, a putative metastasis suppressor protein, is implicated in actin cytoskeletal control and interaction with a protein tyrosine phosphatase (PTP). MIM was originally described as a protein whose mRNA was Missing in Metastasis, as it was found not to be present in metastatic bladder carcinoma cell lines [Lee, Y.G., Macoska, J.A., Korenchuk, S. and Pienta, K.J. (2002) Neoplasia 4, 291–294]. We further characterized a variant of MIM, which we call MIM-B, and which we believe may be a link between tyrosine kinase signalling and the actin cytoskeleton. We have shown, using purified proteins and cell extracts, that MIM-B is an actin-binding protein, probably via a WASP (Wiskott–Aldrich syndrome protein)-homology 2 domain at its C-terminus. We have also found that MIM-B binds to the cytoplasmic domain of receptor PTPδ. Expression of full-length MIM-B induces actin-rich protrusions resembling microspikes and lamellipodia at the plasma membrane and promotes disassembly of actin stress fibres. The C-terminal portion of MIM-B is localized in the cytoplasm and does not affect the actin cytoskeleton when expressed, while the N-terminal portion localizes to internal vesicles and probably targets the protein to membranes. We postulate that MIM-B may be a regulator of actin assembly downstream of tyrosine kinase signalling and that this activity may explain the involvement of MIM in the metastasis of cancer cells.

  • actin
  • cytoskeleton
  • metastasis
  • motility
  • phosphatase
  • signalling

Footnotes

  • 1 These authors contributed equally to this work.

  • Abbreviations used: DLAR, Drosophila leukocyte common antigen-related; GST, glutathione-S-transferase; MIM, missing in metastasis; RPTP, receptor protein tyrosine phosphatase; RPTPδ-CD, cytoplasmic domain of RPTPδ; WASP, Wiskott–Aldrich syndrome protein; WH2, WASP-homology 2.