Glucokinase (GK) is a key enzyme for glucose utilization in liver and shows a higher expression in the perivenous zone. In primary rat hepatocytes, the GK gene expression was activated by HNF (hepatic nuclear factor)-4α via the sequence −52/−39 of the GK promoter. Venous pO2 enhanced HNF-4 levels and HNF-4 binding to the GK—HNF-4 element. Thus, HNF-4α could play the role of a regulator for zonated GK expression.
- maturity-onset diabetes of the young
- metabolic zonation
- nuclear receptor
↵1 Deceased 10 May 2002
Abbreviations used: DIG, digoxigenin; EMSA, electrophoretic mobility-shift assay; EPO, erythropoietin; GK, glucokinase; HNF, hepatic nuclear factor; LUC, luciferase; MODY, maturity-onset diabetes of the young; PKL, pyruvate kinase type liver; WT, wild-type.
- The Biochemical Society, London ©2002