We have determined the structure of a polysaccharide from strain B33, a fast-growing bacterium that forms nitrogen-fixing nodules with Asiatic and American soya bean cultivars. On the basis of monosaccharide analysis, methylation analysis, one-dimensional 1H- and 13C-NMR and two-dimensional NMR experiments, the structure was shown to consist of a polymer having the repeating unit → 6)-4-O-methyl-α-d-Glcp-(1 → 4)-3-O-methyl-β-d-GlcpA-(1 → (where GlcpA is glucopyranuronic acid and Glcp is glucopyranose). Strain B33 produces a K-antigen polysaccharide repeating unit that does not have the structural motif sugar-Kdx [where Kdx is 3-deoxy-d-manno-2-octulosonic acid (Kdo) or a Kdo-related acid] proposed for different Sinorhizobium fredii strains, all of them being effective with Asiatic soya bean cultivars but unable to form nitrogen-fixing nodules with American soya bean cultivars. Instead, it resembles the K-antigen of S. fredii strain HH303 (rhamnose, galacturonic acid)n, which is also effective with both groups of soya bean cultivars. Only the capsular polysaccharide from strains B33 and HH303have monosaccharide components that are also present in the surface polysaccharide of Bradyrhizobium elkanii strains, which consists of a 4-O-methyl-d-glucurono-l-rhamnan.
- chemical structure
- K-antigen polysaccharide
- soya bean rhizobia
Abbreviations used: FSR, fast-growing soya bean rhizobium; LPS, lipopolysaccharide; KPS, capsular polysaccharide; HSQC, 1H-detection mode via single-quantum coherence; HMBC, heteronuclear multiple-bond correlation; NOESY, nuclear Overhauser effect spectroscopy; Kdo, 3-deoxy-d-manno-2-octulosonic acid; 1D, one-dimensional; 2D, two-dimensional; Kdx, 1-carboxy-2-keto-3-deoxysugar; GlcpA, glucopyranuronic acid; Glcp, glucopyranose.
- The Biochemical Society, London ©2001