The precursor protein translocase of the mitochondrial outer membrane (Tom) is a multi-subunit complex containing receptors and a general import channel, of which the core component is Tom40p. Nuclear-encoded mitochondrial precursor proteins are first recognized by surface receptors and then pass through the import channel. The Tom complex has been purified; however, the protein–protein interactions that drive its assembly and maintain its stability have been difficult to study. Here we show that Saccharomyces cerevisiae Tom40p expressed in bacteria and purified to homogeneity associates efficiently with itself. The self-association is very strong and can withstand up to 4M urea or 1M salt. The tight self-association does not require the N-terminal segment of Tom40p. Furthermore, purified Tom40p preferentially recognizes the targeting sequence of mitochondrial precursor proteins. Although the binding of the targeting sequence to Tom40p is inhibited by urea concentrations in excess of 1M, it is moderately resistant to 1M salt. Simultaneous self-assembly and precursor protein binding suggest that Tom40p contains at least two different domains mediating these processes. The experimental approach described here should be useful for analysing protein–protein interactions involving individual or groups of components of the mitochondrial import machinery.
- protein translocase
- signal sequence binding
- stability of protein–protein interactions
Abbreviations used: Tom, translocase of outer membrane; ΔNTom40p, Tom40p in which the first 93 amino acids have been deleted; Yfh1p, yeast frataxin homologue; pYfh1p, precursor of Yfh1p; Δ20Yfh1p, Yfh1p lacking the first 20 amino acids; mYfh1p, mature Yfh1p, in which the entire 51-amino-acid signal sequence of Yfh1p has been deleted; Yfh1p-(1–22)–Protein A, protein in which the first 22 amino acids of Yfh1p are linked to Protein A; Ni-NTA, Ni2+-nitrilotriacetate.
- The Biochemical Society, London ©2001