Effects of ischaemia-reperfusion injury (I/R) of liver on expression of rat glutathione S-transferase (rGST) isoenzymes that metabolize products of oxidative stress were examined. Rats underwent lobar liver ischaemia for 30 min followed by reperfusion. In ischaemic lobes, rGSTA1/A2 transcript levels increased significantly 12 h after I/R (2.94-fold) and protein levels increased significantly at 24 h (1.45-fold); increased transcript levels were also observed in nonischaemic lobes (1.78-fold). Superoxide dismutase prevented I/R and the increases in transcript and protein levels in ischaemic and non-ischaemic lobes. By in-situ hybridization, increases in transcript levels at 6 h were present in zones 2 and 3 of the ischaemic lobes and peaked at 12 h (2.5-fold zone 2, 4.5-fold zone 3). Significant increases in transcript levels also were observed at 24 h in zones 2 (2.0-fold) and 3 (2.9-fold) of non-ischaemic lobes. Nuclear run-off assays showed a 1.8-fold increase in rGSTA1/A2 transcription rates in ischaemic lobes at 3 h. We conclude that I/R causes increased rGSTA1/A2 expression in the zone of the hepatic lobule most susceptible to oxidative injury and that this expression may be an important defence against injury.
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February 1998
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Research Article|
February 15 1998
Ischaemia and reperfusion injury of rat liver increases expression of glutathione S-transferase A1/A2 in zone 3 of the hepatic lobule
D. Gene BRANUM;
D. Gene BRANUM
*Department of Surgery, Emory University School of Medicine, 2101 Woodruff Memorial Building, 1639 Pierce Drive, Atlanta, GA 30322, U.S.A.
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Niazy SELIM;
Niazy SELIM
*Department of Surgery, Emory University School of Medicine, 2101 Woodruff Memorial Building, 1639 Pierce Drive, Atlanta, GA 30322, U.S.A.
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Xia LIU;
Xia LIU
*Department of Surgery, Emory University School of Medicine, 2101 Woodruff Memorial Building, 1639 Pierce Drive, Atlanta, GA 30322, U.S.A.
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Richard WHALEN;
Richard WHALEN
†Department of Medicine, Emory University School of Medicine, 2101 Woodruff Memorial Building, 1639 Pierce Drive, Atlanta, GA 30322, U.S.A.
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D. Thomas BOYER
D. Thomas BOYER
1
†Department of Medicine, Emory University School of Medicine, 2101 Woodruff Memorial Building, 1639 Pierce Drive, Atlanta, GA 30322, U.S.A.
1To whom correspondence should be addressed.
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Publisher: Portland Press Ltd
Received:
June 27 1997
Revision Received:
September 22 1997
Accepted:
September 25 1997
Online ISSN: 1470-8728
Print ISSN: 0264-6021
The Biochemical Society, London © 1998
1998
Biochem J (1998) 330 (1): 73–79.
Article history
Received:
June 27 1997
Revision Received:
September 22 1997
Accepted:
September 25 1997
Citation
D. Gene BRANUM, Niazy SELIM, Xia LIU, Richard WHALEN, D. Thomas BOYER; Ischaemia and reperfusion injury of rat liver increases expression of glutathione S-transferase A1/A2 in zone 3 of the hepatic lobule. Biochem J 15 February 1998; 330 (1): 73–79. doi: https://doi.org/10.1042/bj3300073
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