Biochemical Journal

Research communication

Rac1 is required for cell proliferation and G2/M progression

Kimberly A. MOORE, Rachna SETHI, A. Masharn DOANES, Thomas M. JOHNSON, John B. PRACYK, Martha KIRBY, Kaikobad IRANI, Pascal J. GOLDSCHMIDT-CLERMONT, Toren FINKEL


We have transiently expressed a dominant negative form of rac1 (N17rac1) using adenoviral-mediated gene transfer. The level of N17rac1 expression is demonstrated to be proportional to the multiplicity of infection. Expression of N17rac1 in Rat 2 fibroblasts results in cytostatic growth arrest. Cell-cycle analysis demonstrates that cells expressing N17rac1 accumulate in G2/M. These results suggest that rac1 is required for cell proliferation and provide the first demonstration in mammalian cells of a role for small GTP-binding proteins in the G2/M transition.