A new fungal immunomodulatory protein (Fip) has been purified from the edible mushroom, Volvariella volvacea, and designated Fip-vvo. Analysis of the purified protein by SDS/PAGE followed by Coomassie Blue staining demonstrated that Fip-vvo is a single polypeptide with an apparent molecular mass of 15 kDa. Periodic acid/Schiff staining showed that this single polypeptide lacks carbohydrates. Using an in vitro bioassay measuring blast-formation stimulatory activity, Fip-vvo was shown to stimulate the maximum proliferation of human peripheral blood lymphocytes at a concentration of 5 μg/ml. Fip-vvo was capable of agglutinating rat red blood cells. Neither haemagglutination nor mitogenic activities were inhibited by mono- or dimeric sugars. In vivo, repeat administration of Fip-vvo greatly reduced the production of BSA-induced Arthus reaction in mice, whereas little effect was observed on the prevention of systemic anaphylaxis reactions. The selectively enhanced transcriptional expression of interleukin (IL)-2, IL-4, interferon-γ, tumour necrosis factor-α, lymphotoxin and IL-2 receptor by Fip-vvo was also demonstrated by reverse transcriptase-PCR. This finding suggests that Fip-vvo exerts its immunomodulatory effects via cytokine regulation. In addition, the complete amino acid sequence of Fip-vvo was obtained by direct protein sequencing. This protein consists of 112 amino acid residues with a blocked N-terminal end and has a calculated molecular mass of 12667 Da not including the N-terminal blocking group. By gel filtration analysis, Fip-vvo exhibited a molecular mass of 26 kDa for the native molecules in PBS. This result indicates that native Fip-vvo is most likely a non-covalently associated homodimeric molecule.
Skip Nav Destination
Article navigation
April 1997
-
Cover Image
Cover Image
- PDF Icon PDF LinkFront Matter
- PDF Icon PDF LinkTable of Contents
Research Article|
April 15 1997
Fip-vvo, a new fungal immunomodulatory protein isolated from Volvariella volvacea
Hao-Chi HSU;
Hao-Chi HSU
*Institute of Biochemistry, National Taiwan University, Taipei, Taiwan 100, Republic of China
Search for other works by this author on:
Chyong-Ing HSU;
Chyong-Ing HSU
*Institute of Biochemistry, National Taiwan University, Taipei, Taiwan 100, Republic of China
Search for other works by this author on:
Rong-Hwa LIN;
Rong-Hwa LIN
†Institute of Immunology, National Taiwan University, Taipei, Taiwan 100, Republic of China
Search for other works by this author on:
Chian-Liang KAO;
Chian-Liang KAO
‡Department of Medical Technology, College of Medicine, National Taiwan University, Taipei, Taiwan 100, Republic of China
Search for other works by this author on:
Jung-Yaw LIN
Jung-Yaw LIN
§
*Institute of Biochemistry, National Taiwan University, Taipei, Taiwan 100, Republic of China
§To whom correspondence should be addressed.
Search for other works by this author on:
Publisher: Portland Press Ltd
Received:
September 04 1996
Revision Received:
November 13 1996
Accepted:
December 05 1996
Online ISSN: 1470-8728
Print ISSN: 0264-6021
The Biochemical Society, London © 1997
1997
Biochem J (1997) 323 (2): 557–565.
Article history
Received:
September 04 1996
Revision Received:
November 13 1996
Accepted:
December 05 1996
Citation
Hao-Chi HSU, Chyong-Ing HSU, Rong-Hwa LIN, Chian-Liang KAO, Jung-Yaw LIN; Fip-vvo, a new fungal immunomodulatory protein isolated from Volvariella volvacea. Biochem J 15 April 1997; 323 (2): 557–565. doi: https://doi.org/10.1042/bj3230557
Download citation file:
Sign in
Don't already have an account? Register
Sign in to your personal account
You could not be signed in. Please check your email address / username and password and try again.
Could not validate captcha. Please try again.