Biochemical Journal

Research article

Purification and characterization of sn-1-stearoyl-2-arachidonoylglycerol kinase from pig testes

Matthew N. HODGKIN, Sandra D. GARDNER, Sally ROSE, Andrew PATERSON, Ashley MARTIN, Michael J. O. WAKELAM

Abstract

1-Stearoyl-2-arachidonoylglycerol (SAG) kinase was identified in the particulate fraction of pig testes. This activity was enriched by hydroxyapatite and blue dye chromatography. The enzyme was selective for polyunsaturated diradylglycerol species and activity was not modulated by other diradylglycerol species or sphingomyelin metabolites. Further purification resulted in the isolation of 55 and 50 kDa proteins that corresponded with SAG kinase activity. These results support the view that the phosphorylation of polyunsaturated diradylglycerol is regulated by structural determinants in the molecule.