Research communication

Activation of CPP32 and Mch3α in wild-type p53-induced apoptosis

Julia M. CHANDLER, Emad S. ALNEMRI, Gerald M. COHEN, Marion MacFARLANE


DNA-damaging agents induce apoptosis primarily by a p53-dependent pathway. LTR6 cells containing a temperature-sensitive p53 were used to dissect further the mechanisms of p53-induced apoptosis. Apoptosis was accompanied by the processing and activation of CPP32 and Mch3α, together with the cleavage of poly(ADP-ribose) polymerase and lamin B1. These results demonstrate a critical role for the activation of interleukin-1β-converting enzyme-like proteases in p53-induced apoptosis.