PITALRE is a human protein kinase belonging to the cell division cycle 2 (CDC2) kinase family, and is the catalytic subunit of a multimeric complex that contains several cellular proteins. PITALRE complexes from several cell lines and tissues phosphorylate retinoblastoma protein and myelin basic protein (MBP). In the present work, we have found that MBP is phosphorylated by PITALRE complexes on both Ser and Thr residues. Two different antibodies raised to PITALRE purified virtually identical kinase activities, as analysed by MBP phosphopeptide mapping and phosphoamino acid analysis. We have identified the proline-directed residue Ser-162 of MBP as a major phosphorylation site for PITALRE. In addition, our results suggest that one of the two MBP proline-directed threonine residues, Thr-97, is also selectively phosphorylated by PITALRE. These data, together with analysis of different peptide substrates derived from sites on MBP that are phosphorylated by PITALRE, indicate that PITALRE is a Ser/Thr proline-directed kinase. In addition, our results show that PITALRE has a substrate site specificity distinguishable from those of the CDC2 and cyclin-dependent kinase 2 (CDK2).
Skip Nav Destination
Article navigation
December 1996
-
Cover Image
Cover Image
- PDF Icon PDF LinkFront Matter
- PDF Icon PDF LinkTable of Contents
Research Article|
December 15 1996
Phosphorylation site specificity of the CDC2-related kinase PITALRE
Judit GARRIGA;
Judit GARRIGA
*Fels Institute for Cancer Research and Molecular Biology, Temple University School of Medicine, 3307 North Broad St., Philadelphia, PA 19140, U.S.A.
Search for other works by this author on:
Edy SEGURA;
Edy SEGURA
*Fels Institute for Cancer Research and Molecular Biology, Temple University School of Medicine, 3307 North Broad St., Philadelphia, PA 19140, U.S.A.
Search for other works by this author on:
Xavier MAYOL;
Xavier MAYOL
*Fels Institute for Cancer Research and Molecular Biology, Temple University School of Medicine, 3307 North Broad St., Philadelphia, PA 19140, U.S.A.
Search for other works by this author on:
Charles GRUBMEYER;
Charles GRUBMEYER
*Fels Institute for Cancer Research and Molecular Biology, Temple University School of Medicine, 3307 North Broad St., Philadelphia, PA 19140, U.S.A.
†Department of Biochemistry, Temple University School of Medicine, 3307 North Broad St., Philadelphia, PA 19140, U.S.A.
Search for other works by this author on:
Xavier GRAÑA
Xavier GRAÑA
‡
*Fels Institute for Cancer Research and Molecular Biology, Temple University School of Medicine, 3307 North Broad St., Philadelphia, PA 19140, U.S.A.
‡To whom correspondence should be addressed.
Search for other works by this author on:
Publisher: Portland Press Ltd
Received:
May 28 1996
Revision Received:
August 08 1996
Accepted:
August 30 1996
Online ISSN: 1470-8728
Print ISSN: 0264-6021
The Biochemical Society, London © 1996
1996
Biochem J (1996) 320 (3): 983–989.
Article history
Received:
May 28 1996
Revision Received:
August 08 1996
Accepted:
August 30 1996
Citation
Judit GARRIGA, Edy SEGURA, Xavier MAYOL, Charles GRUBMEYER, Xavier GRAÑA; Phosphorylation site specificity of the CDC2-related kinase PITALRE. Biochem J 15 December 1996; 320 (3): 983–989. doi: https://doi.org/10.1042/bj3200983
Download citation file:
Sign in
Don't already have an account? Register
Sign in to your personal account
You could not be signed in. Please check your email address / username and password and try again.
Could not validate captcha. Please try again.