We have identified a novel human cyclophilin (hCyP-60) which interacts with the proteinase inhibitor eglin c using the yeast two-hybrid system. A cDNA isolated from a Raji B lymphocyte library reveals a domain showing sequence similarity to known cyclophilins flanked by unique N- and C-terminal residues. In addition, hCyP-60 contains a tyrosine residue (Tyr389) instead of a tryptophan residue found in most eukaryotic cyclophilins at a position important for cyclosporin binding. Northern and Western analysis reveal widespread expression with considerable tissue-specific variation. Specifically, the highest levels of mRNA are detected in the thymus, pancreas, testis, and K-562 cell line, while the most protein is detected in the kidney. Immunohistochemistry indicates a nuclear-specific localization both in transfected cells and tissue sections. hCyP-60's specific subcellular localization and conserved amino acid sequence suggest that it may play a specific role in the nucleus.
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Research Article|
February 15 1996
Identification of a nuclear-specific cyclophilin which interacts with the proteinase inhibitor eglin c
Bruce B. WANG;
Bruce B. WANG
1Khepri Pharmaceuticals, 260 Littlefield Avenue, South San Francisco, CA 94080, U.S.A.
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Kirk J. HAYENGA;
Kirk J. HAYENGA
1Khepri Pharmaceuticals, 260 Littlefield Avenue, South San Francisco, CA 94080, U.S.A.
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Donald G. PAYAN;
Donald G. PAYAN
1Khepri Pharmaceuticals, 260 Littlefield Avenue, South San Francisco, CA 94080, U.S.A.
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Joseph M. FISHER
Joseph M. FISHER
*
1Khepri Pharmaceuticals, 260 Littlefield Avenue, South San Francisco, CA 94080, U.S.A.
*To whom correspondence should be sent.
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Publisher: Portland Press Ltd
Received:
July 24 1995
Revision Received:
October 11 1995
Accepted:
October 23 1995
Online ISSN: 1470-8728
Print ISSN: 0264-6021
The Biochemical Society, London © 1996
1996
Biochem J (1996) 314 (1): 313–319.
Article history
Received:
July 24 1995
Revision Received:
October 11 1995
Accepted:
October 23 1995
Citation
Bruce B. WANG, Kirk J. HAYENGA, Donald G. PAYAN, Joseph M. FISHER; Identification of a nuclear-specific cyclophilin which interacts with the proteinase inhibitor eglin c. Biochem J 15 February 1996; 314 (1): 313–319. doi: https://doi.org/10.1042/bj3140313
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