Glutathione S-transferase CL1-2 heterodimers purified from 1-day-old chick livers were digested with Achromobacter proteinase I. The resulting fragments were separated for amino acid sequence analysis. Oligonucleotide probes were constructed based on sequence similarity to class-Theta glutathione S-transferases for PCR using a chicken liver cDNA library as template. A full-length clone (1725 bp) encoding a polypeptide comprising 261 amino acids was isolated. Including conservative substitutions, this protein has 70-73% sequence similarity with other mammalian class-Theta glutathione S-transferases. Based on known X-ray crystal structures of class-Alpha, -Mu and -Pi glutathione S-transferases, a model is constructed for the N-terminal 232 residues of CL1.
Skip Nav Destination
Article navigation
November 1995
-
Cover Image
Cover Image
- PDF Icon PDF LinkFront Matter
- PDF Icon PDF LinkTable of Contents
- PDF Icon PDF LinkAdvertising
Research Article|
November 15 1995
Amino acid sequencing, molecular cloning and modelling of the chick liver class-theta glutathione S-transferase CL1
C D Hsiao;
C D Hsiao
1Institute of Molecular Biology, Academia Sinica, Taipei, Taiwan, R.O.C.
Search for other works by this author on:
E O Martsen;
E O Martsen
1Institute of Molecular Biology, Academia Sinica, Taipei, Taiwan, R.O.C.
Search for other works by this author on:
J Y Lee;
J Y Lee
1Institute of Molecular Biology, Academia Sinica, Taipei, Taiwan, R.O.C.
Search for other works by this author on:
S P Tsai;
S P Tsai
1Institute of Molecular Biology, Academia Sinica, Taipei, Taiwan, R.O.C.
Search for other works by this author on:
M F Tam
M F Tam
1Institute of Molecular Biology, Academia Sinica, Taipei, Taiwan, R.O.C.
Search for other works by this author on:
Publisher: Portland Press Ltd
Online ISSN: 1470-8728
Print ISSN: 0264-6021
© 1995 The Biochemical Society, London
1995
Biochem J (1995) 312 (1): 91–98.
Citation
C D Hsiao, E O Martsen, J Y Lee, S P Tsai, M F Tam; Amino acid sequencing, molecular cloning and modelling of the chick liver class-theta glutathione S-transferase CL1. Biochem J 15 November 1995; 312 (1): 91–98. doi: https://doi.org/10.1042/bj3120091
Download citation file:
Sign in
Don't already have an account? Register
Sign in to your personal account
You could not be signed in. Please check your email address / username and password and try again.
Could not validate captcha. Please try again.