Paraoxonase is a serum protein bound to high-density lipoproteins (HDLs). The physiological function of the enzyme is unknown, but a role in lipid metabolism has been postulated. To date, studies of the protein have had to rely on measurements of enzyme activity with various substrates. We have developed a highly specific, competitive e.l.i.s.a. using a previously characterized monoclonal antibody. The assay can detect 20 ng of paraoxonase with a working range of 75-600 ng. Intra- and interassay coefficients of variation were 6.5 and 7.9% respectively. Serum concentrations of paraoxonase in healthy subjects from Geneva and Manchester ranged from 25 to 118 micrograms/ml. There were significant differences in mean concentrations between the two groups (Geneva, 79.3 +/- 18.7 micrograms/ml; Manchester, 59.9 +/- 24.1 micrograms/ml: P < 0.001), differences also apparent when subjects were compared according to paraoxonase phenotype. These appeared to be largely a consequence of differences in apolipoprotein A-I concentrations between the two populations, suggesting that HDL particle number may be important in determining serum levels of paraoxonase. Paraoxonase specific activities were also significantly different between the two groups of subjects (Geneva, 2.08 +/- 0.96 units/mg; Manchester, 3.08 +/- 1.73 units/mg: P < 0.001), which may reflect differences in HDL particle composition. The e.l.i.s.a. should furnish the necessary complement to studies of paraoxonase enzymic activity and has already provided evidence for differences with respect to serum levels of the protein both between populations and between phenotypes within populations.
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December 1994
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Research Article|
December 01 1994
Quantification of human serum paraoxonase by enzyme-linked immunoassay: population differences in protein concentrations
M C Blatter Garin;
M C Blatter Garin
*Division of Diabetology, Department of Medicine, University Hospital, 24 rue Micheli-du-Crest, 1211 Geneva 14, Switzerland
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C Abbott;
C Abbott
†Department of Medicine, University of Manchester, The Royal Infirmary, Manchester M13 9WL, U.K.
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S Messmer;
S Messmer
*Division of Diabetology, Department of Medicine, University Hospital, 24 rue Micheli-du-Crest, 1211 Geneva 14, Switzerland
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M Mackness;
M Mackness
†Department of Medicine, University of Manchester, The Royal Infirmary, Manchester M13 9WL, U.K.
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P Durrington;
P Durrington
†Department of Medicine, University of Manchester, The Royal Infirmary, Manchester M13 9WL, U.K.
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D Pometta;
D Pometta
*Division of Diabetology, Department of Medicine, University Hospital, 24 rue Micheli-du-Crest, 1211 Geneva 14, Switzerland
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R W James
R W James
*Division of Diabetology, Department of Medicine, University Hospital, 24 rue Micheli-du-Crest, 1211 Geneva 14, Switzerland
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Publisher: Portland Press Ltd
Online ISSN: 1470-8728
Print ISSN: 0264-6021
© 1994 The Biochemical Society, London
1994
Biochem J (1994) 304 (2): 549–554.
Citation
M C Blatter Garin, C Abbott, S Messmer, M Mackness, P Durrington, D Pometta, R W James; Quantification of human serum paraoxonase by enzyme-linked immunoassay: population differences in protein concentrations. Biochem J 1 December 1994; 304 (2): 549–554. doi: https://doi.org/10.1042/bj3040549
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