Protein disulphide isomerase (PDI) is a unique polypeptide which resides in the lumen of the endoplasmic reticulum and also functions as the beta-subunit of prolyl 4-hydroxylase, as a cellular thyroid hormone-binding protein, as the smaller subunit of the microsomal triacylglycerol transfer protein complex, as a dehydroascorbate reductase and as a protein that binds various peptides in a specific manner. We have recently demonstrated that the promoter of the PDI gene contains six CCAAT boxes and other elements which are needed for efficient transcription. We now demonstrate that purified human recombinant transcription factor Sp1 interacts with two perfect GGGCGG sequences and three other GC-rich elements of the PDI promoter. Sp1 also appears to participate in the regulation of PDI gene expression, since overexpression of Sp1 stimulated PDI promoter activity in HeLa cells and mutations introduced into each of these Sp1-binding sites separately reduced the promoter strength, although even the largest decrease was only about 50%. These results support our view that expression of the gene for this polypeptide with multiple functions is secured by several regulatory elements, some of which are functionally redundant.
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May 1993
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Research Article|
May 15 1993
Interaction of transcription factor Sp1 with the promoter of the gene for the multifunctional protein disulphide isomerase polypeptide
K Tasanen;
K Tasanen
1Collagen Research Unit, Biocenter and Department of Medical Biochemistry, University of Oulu, SF-90220 Oulu, Finland
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J Oikarinen;
J Oikarinen
1Collagen Research Unit, Biocenter and Department of Medical Biochemistry, University of Oulu, SF-90220 Oulu, Finland
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K I Kivirikko;
K I Kivirikko
1Collagen Research Unit, Biocenter and Department of Medical Biochemistry, University of Oulu, SF-90220 Oulu, Finland
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T Pihlajaniemi
T Pihlajaniemi
1Collagen Research Unit, Biocenter and Department of Medical Biochemistry, University of Oulu, SF-90220 Oulu, Finland
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Publisher: Portland Press Ltd
Online ISSN: 1470-8728
Print ISSN: 0264-6021
© 1993 The Biochemical Society, London
1993
Biochem J (1993) 292 (1): 41–45.
Citation
K Tasanen, J Oikarinen, K I Kivirikko, T Pihlajaniemi; Interaction of transcription factor Sp1 with the promoter of the gene for the multifunctional protein disulphide isomerase polypeptide. Biochem J 15 May 1993; 292 (1): 41–45. doi: https://doi.org/10.1042/bj2920041
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