The methodology previously developed for determining the Flux Control Coefficients [Delgado & Liao (1992) Biochem. J. 282, 919-927] is extended to the calculation of metabolite Concentration Control Coefficients. It is shown that the transient metabolite concentrations are related by a few algebraic equations, attributed to mass balance, stoichiometric constraints, quasi-equilibrium or quasi-steady states, and kinetic regulations. The coefficients in these relations can be estimated using linear regression, and can be used to calculate the Control Coefficients. The theoretical basis and two examples are discussed. Although the methodology is derived based on the linear approximation of enzyme kinetics, it yields reasonably good estimates of the Control Coefficients for systems with non-linear kinetics.
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August 1992
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Research Article|
August 01 1992
Metabolic control analysis using transient metabolite concentrations. Determination of metabolite concentration control coefficients
J Delgado;
J Delgado
1Department of Chemical Engineering, Texas A&M University, College Station, Texas 77843-3122, U.S.A.
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J C Liao
J C Liao
1Department of Chemical Engineering, Texas A&M University, College Station, Texas 77843-3122, U.S.A.
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Publisher: Portland Press Ltd
Online ISSN: 1470-8728
Print ISSN: 0264-6021
© 1992 The Biochemical Society, London
1992
Biochem J (1992) 285 (3): 965–972.
Citation
J Delgado, J C Liao; Metabolic control analysis using transient metabolite concentrations. Determination of metabolite concentration control coefficients. Biochem J 1 August 1992; 285 (3): 965–972. doi: https://doi.org/10.1042/bj2850965
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