cDNA clones encoding the fatty-acid- biosynthetic enzyme NADPH-linked 3-oxoacyl-(acyl carrier protein) (ACP) reductase were isolated from a Brassica napus (rape) developing seed library and from an Arabidopsis thaliana (thale cress) leaf library. The N-terminal end of the coding region shows features typical of a stromal-targeting plastid-transit peptide. The deduced amino acid sequences have 41% and 55% identity respectively with the nodG-gene product of Rhizobium meliloti, one of the host-specific genes that restrict infectivity of this bacterium to a small range of host plants. The probability that the nodG-gene product is a oxoreductase strengthens the hypothesis that some of the host-specific nod-gene products are enzymes which synthesize polyketides that uniquely modify the Rhizobium nodulation signal molecule.
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April 1992
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Research Article|
April 15 1992
Molecular cloning of higher-plant 3-oxoacyl-(acyl carrier protein) reductase. Sequence identities with the nodG-gene product of the nitrogen-fixing soil bacterium Rhizobium meliloti
A R Slabas;
A R Slabas
*School of Biological Sciences, University of Durham, Durham DHI 3LE, U.K.
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D Chase;
D Chase
*School of Biological Sciences, University of Durham, Durham DHI 3LE, U.K.
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I Nishida;
I Nishida
†National Institute for Basic Biology, Myodaiji, Okazaki 444, Japan
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N Murata;
N Murata
†National Institute for Basic Biology, Myodaiji, Okazaki 444, Japan
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C Sidebottom;
C Sidebottom
‡Biosciences Division, Unilever Research, Bedford MK44 ILQ, U.K.
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R Safford;
R Safford
‡Biosciences Division, Unilever Research, Bedford MK44 ILQ, U.K.
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P S Sheldon;
P S Sheldon
§Department of Biochemistry, University of Birmingham, Birmingham B15 2TT, U.K.
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R G Kekwick;
R G Kekwick
§Department of Biochemistry, University of Birmingham, Birmingham B15 2TT, U.K.
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D G Hardie;
D G Hardie
║Protein Phosphorylation Group, Department of Biochemistry, University of Dundee, Dundee DDI 4HN, Scotland, U.K.
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R W Mackintosh
R W Mackintosh
║Protein Phosphorylation Group, Department of Biochemistry, University of Dundee, Dundee DDI 4HN, Scotland, U.K.
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Publisher: Portland Press Ltd
Online ISSN: 1470-8728
Print ISSN: 0264-6021
© 1992 The Biochemical Society, London
1992
Biochem J (1992) 283 (2): 321–326.
Citation
A R Slabas, D Chase, I Nishida, N Murata, C Sidebottom, R Safford, P S Sheldon, R G Kekwick, D G Hardie, R W Mackintosh; Molecular cloning of higher-plant 3-oxoacyl-(acyl carrier protein) reductase. Sequence identities with the nodG-gene product of the nitrogen-fixing soil bacterium Rhizobium meliloti. Biochem J 15 April 1992; 283 (2): 321–326. doi: https://doi.org/10.1042/bj2830321
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