Using [32P]Pi and [3H]inositol as precursors, we have detected the presence of phosphatidylinositol, phosphatidylinositol 4-phosphate and phosphatidylinositol 4,5-bisphosphate, and their derivatives inositol phosphate, inositol 1,4-bisphosphate and inositol 1,4,5-trisphosphate respectively, in Trypanosoma cruzi epimastigotes. Using digitonin-permeabilized cells it was possible to detect a stimulation in the formation of inositol 1,4,5-trisphosphate and inositol 1,4-bisphosphate as well as an increased generation of diacylglycerol in the presence of 1 mM-CaCl2. These results are consistent with the operation of a functional inositol phosphate/diacylglycerol pathway in T. cruzi, and constitute the first demonstration of the presence and activation of this pathway in a parasitic protozoan. These results also indicate that this pathway is conserved during evolution from lower to higher eukaryotic organisms.
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April 1991
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Research Article|
April 15 1991
The inositol phosphate/diacylglycerol signalling pathway in Trypanosoma cruzi
R Docampo;
R Docampo
1Department of Veterinary Pathobiology, University of Illinois, 2001 S. Lincoln Ave., Urbana, IL 61801, U.S.A.
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O P Pignataro
O P Pignataro
1Department of Veterinary Pathobiology, University of Illinois, 2001 S. Lincoln Ave., Urbana, IL 61801, U.S.A.
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Publisher: Portland Press Ltd
Online ISSN: 1470-8728
Print ISSN: 0264-6021
© 1991 The Biochemical Society, London
1991
Biochem J (1991) 275 (2): 407–411.
Citation
R Docampo, O P Pignataro; The inositol phosphate/diacylglycerol signalling pathway in Trypanosoma cruzi. Biochem J 15 April 1991; 275 (2): 407–411. doi: https://doi.org/10.1042/bj2750407
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