A hypertrehalosaemic neuropeptide from the corpora cardiaca of the blowfly Phormia terraenovae has been isolated by reversed-phase h.p.l.c., and its primary structure was determined by pulsed-liquid phase sequencing employing Edman chemistry after enzymically deblocking the N-terminal pyroglutamate residue. The C-terminus was also blocked, as indicated by the lack of digestion when the peptide was incubated with carboxypeptidase A. The octapeptide has the sequence pGlu-Leu-Thr-Phe-Ser-Pro-Asp-Trp-NH2 and is clearly defined as a novel member of the RPCH/AKH (red-pigment-concentrating hormone/adipokinetic hormone) family of peptides. It is the first charged member of this family to be found. The synthetic peptide causes an increase in the haemolymph carbohydrate concentration in a dose-dependent fashion in blowflies and therefore is named ‘Phormia terraenovae hypertrehalosaemic hormone’ (Pht-HrTH). In addition, receptors in the fat-body of the American cockroach (Periplaneta americana) recognize the peptide, resulting in carbohydrate elevation in the blood. However, fat-body receptors of the migratory locust (Locusta migratoria) do not recognize this charged molecule, and thus no lipid mobilization is observed in this species.
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July 1990
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Research Article|
July 15 1990
Isolation and structure of a novel charged member of the red–pigment-concentrating hormone-adipokinetic hormone family of peptides isolated from the corpora cardiaca of the blowfly Phormia terraenovae (Diptera)
G Gäde;
G Gäde
*Institut für Zoologie, Lehrstuhl für Tierphysiologie, Heinrich-Heine-Universität, Düsseldorf, Universitätsstr. 1, Gebäude 26.12/00, D-4000 Düsseldorf
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H Wilps;
H Wilps
†Institut für Biologie I (Zoologie), Universität Freiburg, Albertstr.21a, D-7800 Freiburg
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R Kellner
R Kellner
‡Applied Biosystems G.m.b.H., Robert-Koch-Str.16, D-6108 Weiterstadt, Federal Republic of Germany
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Publisher: Portland Press Ltd
Online ISSN: 1470-8728
Print ISSN: 0264-6021
© 1990 London: The Biochemical Society
1990
Biochem J (1990) 269 (2): 309–313.
Citation
G Gäde, H Wilps, R Kellner; Isolation and structure of a novel charged member of the red–pigment-concentrating hormone-adipokinetic hormone family of peptides isolated from the corpora cardiaca of the blowfly Phormia terraenovae (Diptera). Biochem J 15 July 1990; 269 (2): 309–313. doi: https://doi.org/10.1042/bj2690309
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