The degradation of 2-deoxyribose to thiobarbituric acid-reactive material was investigated with two hydroxyl-radical-generating systems: (i) a defined gamma-radiolysis method and (ii) incubation with FeSO4 in phosphate buffer. In each case the thiobarbituric acid-reactive material can be accounted for by malondialdehyde, as measured by an h.p.l.c. method for free malondialdehyde. In the radiolysis system there is a large post-irradiation increase in free malondialdehyde if iron ions are added to the samples. It is proposed that this is due to iron ions catalysing the formation of hydroxyl radicals from radiolytically generated H2O2 as well as stimulating the breakdown of an intermediate deoxyribose degradation product. A mechanism for the formation of malondialdehyde during deoxyribose degradation is proposed.
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June 1988
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Research Article|
June 15 1988
Hydroxyl-radical-induced iron-catalysed degradation of 2-deoxyribose. Quantitative determination of malondialdehyde
K H Cheeseman;
K H Cheeseman
*Department of Biochemistry, Brunel University, Uxbridge, Middx. UB8 3PH, U.K.
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A Beavis;
A Beavis
*Department of Biochemistry, Brunel University, Uxbridge, Middx. UB8 3PH, U.K.
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H Esterbauer
H Esterbauer
†lnstitute of Biochemistry, Karl Franzens University, Schubertstrasse 1, A-8010 Graz, Austria
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Publisher: Portland Press Ltd
Online ISSN: 1470-8728
Print ISSN: 0264-6021
© 1988 London: The Biochemical Society
1988
Biochem J (1988) 252 (3): 649–653.
Citation
K H Cheeseman, A Beavis, H Esterbauer; Hydroxyl-radical-induced iron-catalysed degradation of 2-deoxyribose. Quantitative determination of malondialdehyde. Biochem J 15 June 1988; 252 (3): 649–653. doi: https://doi.org/10.1042/bj2520649
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