Chronic exposure of 3T3-L1 pre-adipocytes to dexamethasone plus 3-isobutyl-1-methylxanthine (IBMX) with or without insulin caused a significant increase in the specific activity of ‘total’ pyruvate dehydrogenase complex (PDC) and in the percentage of the ‘active’ form of the complex compared with cells exposed to a chronic insulin treatment or an acute treatment (2 days) with dexamethasone plus IBMX. In acute-drug-switch-over experiments, dexamethasone also caused an increase in the percentage of ‘active’ PDC in 3T3-L1 adipocytes. The results show that, in 3T3-L1 adipocytes, dexamethasone, even in the absence of insulin, increases the proportion of PDC in its ‘active’ form. The mechanism of the dexamethasone effect remains to be investigated.
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March 1985
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Research Article|
March 01 1985
Modulation by dexamethasone of the pyruvate dehydrogenase-complex activity in 3T3-L1 adipocytes
Publisher: Portland Press Ltd
Online ISSN: 1470-8728
Print ISSN: 0264-6021
© 1985 London: The Biochemical Society
1985
Biochem J (1985) 226 (2): 607–611.
Citation
M S Patel, C Raefsky, C W C Hu, L Ho; Modulation by dexamethasone of the pyruvate dehydrogenase-complex activity in 3T3-L1 adipocytes. Biochem J 1 March 1985; 226 (2): 607–611. doi: https://doi.org/10.1042/bj2260607
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